Evidence map›Paper›PMID 28548373›Full record

ArticleMolecular oral microbiology2017

Role of de-N-acetylase PgaB from Aggregatibacter actinomycetemcomitans in exopolysaccharide export in biofilm mode of growth.

M Shanmugam, A O Oyeniyi, C Parthiban, S K Gujjarlapudi, G B Pier, N Ramasubbu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular oral microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Journal of oral microbiology · 2024
    Article
  3. Article
  4. Frontiers in microbiology · 2022
    Article
  5. The dental plaque biofilm matrix.Periodontology 2000 · 2021
    Review
  6. Frontiers in microbiology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

M ShanmugamDepartment of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA.
A O OyeniyiDepartment of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA.
C ParthibanDepartment of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA.
S K GujjarlapudiDepartment of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA.
G B PierDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
N RamasubbuDepartment of Oral Biology, Rutgers School of Dental Medicine, Newark, NJ, USA.ORCID 0000-0003-3198-8296
Rutgers, The State University of New Jersey · USBrigham and Women's Hospital · US

Funding

PGA exopolysaccharide in biofilm formation and pathogenicity of Aggregatibacter aR01DE022544 · NIDCR · UNIV OF MED/DENT OF NJ-NJ DENTAL SCHOOL · PI RAMASUBBU, NARAYANAN, SCHREINER, HELEN CAROL · 2013 to 2016
$1.4M
NIDCR NIH HHS R01 DE022544
6 · The paper itself

Abstract

Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, is the causative agent of localized aggressive periodontitis. Attachment to a biotic surface is a critical first step in the A. actinomycetemcomitans infection process for which exopolysaccharides have been shown to be essential. In addition, the pga operon, containing genes encoding for biosynthetic proteins for poly-N-acetyl glucosamine (PNAG), plays a key role in A. actinomycetemcomitans virulence, as a mutant strain lacking the pga operon induces significantly less bone resorption. Among the genes in the pga operon, pgaB codes for a de-N-acetylase that is responsible for the deacetylation of the PNAG exopolysaccharide. Here we report the role of PgaB in regulation of virulence genes using a markerless, scarless deletion mutant targeting the coding region of the N-terminal catalytic domain of PgaB. The results demonstrate that the N-terminal, catalytic domain of PgaB is crucial for exopolysaccharide export.

Indexed as

AcetylesteraseAcetylglucosamineAggregatibacter actinomycetemcomitansBacterial ProteinsBiofilmsCatalytic DomainGene Expression ProfilingGene Expression Regulation, BacterialGenes, BacterialOpen Reading FramesOperonPeriodontitisPolysaccharides, BacterialSequence DeletionVirulenceAcetylesteraseAcetylglucosamineBacterial Proteinspoly-N-acetyl glucosaminePolysaccharides, Bacterialbiofilmdeacetylationexopolysaccharideexportoral pathogenvirulence

Identifiers

PMID28548373
PMCPMC6118124
OpenAlexW2617129049

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.