ArticleThe Journal of general virology2017
Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
Article in The Journal of general virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius.PLoS pathogens · 2025Article
- Marek's disease: A global challenge to poultry health and productivity.Open veterinary journal · 2025Review
- Article
- Zinc Finger Protein BCL11A Contributes to the Abortive Infection ofJournal of virology · 2022Article
- The Role of Dendritic Cells in the Host Response to Marek's Disease Virus (MDV) as Shown by Transcriptomic Analysis of Susceptible and Resistant Birds.Pathogens (Basel, Switzerland) · 2022Article
- Visualization of Marek's Disease Virus Genomes in Living Cells during Lytic Replication and Latency.Viruses · 2022Article
- ER stress-related molecules induced by Hantaan virus infection in differentiated THP-1 cells.Cell stress & chaperones · 2021Article
- Revisiting cellular immune response to oncogenic Marek's disease virus: the rising of avian T-cell immunity.Cellular and molecular life sciences : CMLS · 2020Review
- Article
- Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4Vaccines · 2020Article
- Review
- Marek's Disease Virus Infection of Natural Killer Cells.Microorganisms · 2019Article
- Article
- Unraveling the role of B cells in the pathogenesis of an oncogenic avian herpesvirus.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Low pathogenic avian influenza virus infection increases the staining intensity of KUL01+ cells including macrophages yet decrease of the staining intensity of KUL01+ cells using clodronate liposomes did not affect the viral genome loads in chickens.Veterinary immunology and immunopathology · 2018Article
- Identification of the Receptor and Cellular Ortholog of the Marek's Disease Virus (MDV) CXC Chemokine.Frontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells. Chicken bone marrow cells were cultured with chicken CSF-1 or chicken IL-4 and chicken CSF-2 for 4 days to produce macrophages and DCs, respectively, and then co-cultured with FACS-sorted chicken embryo fibroblasts (CEFs) infected with recombinant MDV expressing EGFP. Infected phagocytes were identified and sorted by FACS using EGFP expression and phagocyte-specific mAbs. Detection of MDV-specific transcripts of ICP4 (immediate early), pp38 (early), gB (late) and Meq by RT-PCR provided evidence for MDV replication in the infected phagocytes. Time-lapse confocal microscopy was also used to demonstrate MDV spread in these cells. Subsequent co-culture of infected macrophages with CEFs suggests that productive virus infection may occur in these cell types. This is the first report of in vitro infection of phagocytic cells by MDV.
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