ArticleTransgenic research2017
MsmiR156 affects global gene expression and promotes root regenerative capacity and nitrogen fixation activity in alfalfa.
Article in Transgenic research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Genome-wide identification and expression pattern analysis under drought stress of PHD family genes in alfalfa (Medicago sativa).BMC genomics · 2025Article
- Transcriptome-Based miR156-Mediated Expression Dynamics of SPL Transcription Factors During Vegetative to Reproductive Transition in Spinach.Plants (Basel, Switzerland) · 2025Article
- Adventitious Root Formation in Cuttings: Insights fromBiomolecules · 2025Review
- Optimizing nitrogen use efficiency in forest plantations: mechanistic insights fromForestry research · 2025Review
- Article
- TheInternational journal of molecular sciences · 2023Article
- Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa.Plant molecular biology · 2022Article
- SPL12 RegulatesPlants (Basel, Switzerland) · 2022Article
- Identification of Low-Nitrogen-Related miRNAs and Their Target Genes in Sugarcane and the Role ofInternational journal of molecular sciences · 2022Article
- Expression Profiles and Characteristics of Apple lncRNAs in Roots, Phloem, Leaves, Flowers, and Fruit.International journal of molecular sciences · 2022Article
- The miR156b-GmSPL9d module modulates nodulation by targeting multiple core nodulation genes in soybean.The New phytologist · 2022Article
- Roles of non-coding RNAs in the hormonal and nutritional regulation in nodulation and nitrogen fixation.Frontiers in plant science · 2022Review
- Physiological and transcriptome analyses highlight multiple pathways involved in drought stress in Medicago falcata.PloS one · 2022Article
- Genome-Wide Analysis of Long Non-coding RNAs Involved in Nodule Senescence inFrontiers in plant science · 2022Article
- Molecular Bases for the Regulation of Adventitious Root Generation in Plants.Frontiers in plant science · 2021Review
- The Control of Developmental Phase Transitions by microRNAs and Their Targets in Seed Plants.International journal of molecular sciences · 2020Review
- Biotechnological Perspectives of Omics and Genetic Engineering Methods in Alfalfa.Frontiers in plant science · 2020Review
- AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration.The Plant cell · 2020Article
- OsSPL3, an SBP-Domain Protein, Regulates Crown Root Development in Rice.The Plant cell · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNA156 (miR156) regulates a network of downstream genes to affect plant growth and development. We previously generated alfalfa (Medicago sativa) plants that overexpress homologous miR156 (MsmiR156OE), and identified three of its SPL target genes. These plants exhibited increased vegetative yield, delayed flowering and longer roots. In this study, we aimed to elucidate the effect of miR156 on the root system, including effect on nodulation and nitrogen fixation. We found that MsmiR156 overexpression increases root regeneration capacity in alfalfa, but with little effect on root biomass at the early stages of root development. MsmiR156 also promotes nitrogen fixation activity by upregulating expression of nitrogenase-related genes FixK, NifA and RpoH in roots inoculated with Sinorrhizobium meliloti. Furthermore, we conducted transcriptomics analysis of MsmiR156OE alfalfa roots and identified differentially expressed genes belonging to 132 different functional categories, including plant cell wall organization, peptidyl-hypusine synthesis, and response to water stress. Expression analysis also revealed miR156 effects on genes involved in nodulation, root development and phytohormone biosynthesis. The present findings suggest that miR156 regulates root development and nitrogen fixation activity. Taken together, these findings highlight the important role that miR156 may play as a tool in the biotechnological improvement of alfalfa, and potentially other crops.
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