ArticleJournal of industrial microbiology & biotechnology2017
Metabolic engineering of Saccharomyces cerevisiae for production of germacrene A, a precursor of beta-elemene.
Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Reprogramming product selectivity and activity in promiscuous terpene synthases via substrate conformation engineering.Nature communications · 2026Article
- EngineeringSynthetic and systems biotechnology · 2025Article
- Exploration and mutagenesis of the germacrene A synthase fromSynthetic and systems biotechnology · 2025Article
- Characterization and functional reconstruction of a highly productive germacrene A synthase from Liriodendron chinense.Plant biotechnology journal · 2025Article
- Multi-Omics and Functional Insights into Triterpenoid Biosynthesis Pathways inPlants (Basel, Switzerland) · 2025Article
- High-level biosynthesis of enantiopure germacrene D in yeast.Applied microbiology and biotechnology · 2024Article
- Engineering strategies for enhanced 1', 4'-trans-ABA diol production by Botrytis cinerea.Microbial cell factories · 2024Article
- Recent advances in lycopene and germacrene a biosynthesis and their role as antineoplastic drugs.World journal of microbiology & biotechnology · 2024Review
- Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2024Article
- An Investigation of the JAZ Family and the CwMYC2-like Protein to Reveal Their Regulation Roles in the MeJA-Induced Biosynthesis of β-Elemene inInternational journal of molecular sciences · 2023Article
- Switching carbon metabolic flux for enhancing the production of sesquiterpene-based high-density biofuel precursor in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene.Bioresources and bioprocessing · 2023Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Protein Engineering of a Germacrene A Synthase FromFrontiers in plant science · 2022Article
- Metabolic engineering ofMetabolic engineering communications · 2021Article
- Histochemical and Phytochemical Analysis ofMolecules (Basel, Switzerland) · 2021Article
- Improved production of germacrene A, a direct precursor of ß-elemene, in engineered Saccharomyces cerevisiae by expressing a cyanobacterial germacrene A synthase.Microbial cell factories · 2021Article
- Successful Enzyme Colocalization Strategies in Yeast for Increased Synthesis of Non-native Products.Frontiers in bioengineering and biotechnology · 2021Review
- Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Review
- Combinatorial Metabolic Engineering inBioengineering (Basel, Switzerland) · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Beta-elemene, a sesquiterpene and the major component of the medicinal herb Curcuma wenyujin, has antitumor activity against various types of cancer and could potentially serve as a potent antineoplastic drug. However, its current mode of production through extraction from plants has been inefficient and suffers from limited natural resources. Here, we engineered a yeast cell factory for the sustainable production of germacrene A, which can be transformed to beta-elemene by a one-step chemical reaction in vitro. Two heterologous germacrene A synthases (GASs) converting farnesyl pyrophosphate (FPP) to germacrene A were evaluated in yeast for their ability to produce germacrene A. Thereafter, several metabolic engineering strategies were used to improve the production level. Overexpression of truncated 3-hydroxyl-3-methylglutaryl-CoA reductase and fusion of FPP synthase with GAS, led to a sixfold increase in germacrene A production in shake-flask culture. Finally, 190.7 mg/l of germacrene A was achieved. The results reported in this study represent the highest titer of germacrene A reported to date. These results provide a basis for creating an efficient route for further industrial application re-placing the traditional extraction of beta-elemene from plant sources.
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