ArticleParasites & vectors2017
Sources of variability in the measurement of Ascaris lumbricoides infection intensity by Kato-Katz and qPCR.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Systematic review of studies generating individual participant data on the efficacy of drugs for treating soil-transmitted helminthiases and the case for data-sharing.PLoS neglected tropical diseases · 2017Pooled it
- Therapeutic efficacy of albendazole against soil-transmitted helminthiasis in children measured by five diagnostic methods.PLoS neglected tropical diseases · 2019Trial
- Assessment of enteric helminth parasites in bushmeat in Ghana.International journal for parasitology. Parasites and wildlife · 2024Article
- Evaluation of Kato-Katz and multiplex quantitative polymerase chain reaction performance for clinical helminth infections in Thailand using a latent class analysis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Impact of Key Assumptions About the Population Biology of Soil-Transmitted Helminths on the Sustainable Control of Morbidity.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Microscopic and molecular evaluation of Strongyloides venezuelensis in an experimental life cycle using Wistar rats.Biomedica : revista del Instituto Nacional de Salud · 2021Article
- Article
- A novel quantitative real-time PCR diagnostic assay for fecal and nasal swab detection of an otariid lungworm,International journal for parasitology. Parasites and wildlife · 2020Article
- The increased sensitivity of qPCR in comparison to Kato-Katz is required for the accurate assessment of the prevalence of soil-transmitted helminth infection in settings that have received multiple rounds of mass drug administration.Parasites & vectors · 2020Article
- Diagnostic Techniques of Soil-Transmitted Helminths: Impact on Control Measures.Tropical medicine and infectious disease · 2020Review
- Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh.PLoS neglected tropical diseases · 2020Article
- What does soil-transmitted helminth elimination look like? Results from a targeted molecular detection survey in Japan.Parasites & vectors · 2020Article
- The Geshiyaro Project: a study protocol for developing a scalable model of interventions for moving towards the interruption of the transmission of soil-transmitted helminths and schistosome infections in the Wolaita zone of Ethiopia.Parasites & vectors · 2019Article
- Mini-FLOTAC as an alternative, non-invasive diagnostic tool for Schistosoma mansoni and other trematode infections in wildlife reservoirs.Parasites & vectors · 2019Article
- Soil-transmitted helminth reinfection four and six months after mass drug administration: results from the delta region of Myanmar.PLoS neglected tropical diseases · 2019Article
- Insights from quantitative analysis and mathematical modelling on the proposed WHO 2030 goals for soil-transmitted helminths.Gates open research · 2019Article
- A Case for Using Genomics and a Bioinformatics Pipeline to Develop Sensitive and Species-Specific PCR-Based Diagnostics for Soil-Transmitted Helminths.Frontiers in genetics · 2019Review
- Long Term School Based Deworming against Soil-Transmitted Helminths Also Benefits the Untreated Adult Population: Results from a Community-Wide Cross Sectional Survey.Journal of tropical medicine · 2019Article
- Defining stopping criteria for ending randomized clinical trials that investigate the interruption of transmission of soil-transmitted helminths employing mass drug administration.PLoS neglected tropical diseases · 2018Article
- The optimal timing of post-treatment sampling for the assessment of anthelminthic drug efficacy against Ascaris infections in humans.International journal for parasitology. Drugs and drug resistance · 2018Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundUnderstanding and quantifying the sources and implications of error in the measurement of helminth egg intensity using Kato-Katz (KK) and the newly emerging "gold standard" quantitative polymerase chain reaction (qPCR) technique is necessary for the appropriate design of epidemiological studies, including impact assessments for deworming programs.
methodsRepeated measurements of Ascaris lumbricoides infection intensity were made from samples collected in western Kenya using the qPCR and KK techniques. These data were combined with data on post-treatment worm expulsions. Random effects regression models were used to quantify the variability associated with different technical and biological factors for qPCR and KK diagnosis. The relative precision of these methods was compared, as was the precision of multiple qPCR replicates.
resultsFor both KK and qPCR, intensity measurements were largely determined by the identity of the stool donor. Stool donor explained 92.4% of variability in qPCR measurements and 54.5% of observed measurement variance for KK. An additional 39.1% of variance in KK measurements was attributable to having expelled adult A. lumbricoides worms following anthelmintic treatment. For qPCR, the remaining 7.6% of variability was explained by the efficiency of the DNA extraction (2.4%), plate-to-plate variability (0.2%) and other residual factors (5%). Differences in replicate measurements by qPCR were comparatively small. In addition to KK variability based on stool donor infection levels, the slide reader was highly statistically significant, although it only explained 1.4% of the total variation. In a comparison of qPCR and KK variance to mean ratios under ideal conditions, the coefficient of variation was on average 3.6 times larger for KK highlighting increased precision of qPCR.
conclusionsPerson-to-person differences explain the majority of variability in egg intensity measurements by qPCR and KK, with very little additional variability explained by the technical factors associated with the practical implementation of these techniques. qPCR provides approximately 3.6 times more precision in estimating A. lumbricoides egg intensity than KK, and could potentially be made more cost-effective by testing each sample only once without diminishing the power of a study to assess population-level intensity and prevalence.
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