ArticleGenome biology2017
Optimizing complex phenotypes through model-guided multiplex genome engineering.
Article in Genome biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-codedRSC chemical biology · 2026Article
- Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection ofACS synthetic biology · 2026Article
- Engineered orthogonal translation systems from metagenomic libraries expand the genetic code.bioRxiv : the preprint server for biology · 2025Article
- Article
- Expanding the genetic code: In vivo approaches for incorporating non-proteinogenic monomers.Journal of microbiology (Seoul, Korea) · 2025Review
- Genomically recodedbioRxiv : the preprint server for biology · 2024Article
- Native Aminoacyl-tRNA Synthetase/tRNA Pair Drives Highly Efficient Noncanonical Amino Acid Incorporation inACS chemical biology · 2024Article
- Engineered mRNA-ribosome fusions for facile biosynthesis of selenoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Genetic Code Expansion in the Engineered Organism Vmax X2: High Yield and Exceptional Fidelity.ACS central science · 2021Article
- Enhancement of Activity and Thermostability of Keratinase FromFrontiers in bioengineering and biotechnology · 2021Article
- Comparative Analyses of the Transcriptome and Proteome ofFrontiers in microbiology · 2021Article
- Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains.Nature biotechnology · 2018Article
- Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids.Nature communications · 2018Article
- Adaptive evolution of genomically recodedProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Rewriting the Genetic Code.Annual review of microbiology · 2017Review
- Millstone: software for multiplex microbial genome analysis and engineering.Genome biology · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We apply our method to identify six single nucleotide mutations that recover 59% of the fitness defect exhibited by the 63-codon E. coli strain C321.∆A. By introducing targeted combinations of changes in multiplex we generate rich genotypic and phenotypic diversity and characterize clones using whole-genome sequencing and doubling time measurements. Regularized multivariate linear regression accurately quantifies individual allelic effects and overcomes bias from hitchhiking mutations and context-dependence of genome editing efficiency that would confound other strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.