Evidence map›Paper›PMID 28545392›Full record

ArticleBMC cell biology2017

Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis.

Satarupa Das, Joshua M Parker, Can Guven, Weiye Wang, Paul W Kriebel, Wolfgang Losert, Daniel R Larson, Carole A Parent

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Cell interactions in collective cell migration.Development (Cambridge, England) · 2019
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Satarupa DasLaboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
Joshua M ParkerInstitute for Physical Science and Technology, Department of Physics, University of Maryland, College Park, MD, 20742, USA.
Can GuvenInstitute for Physical Science and Technology, Department of Physics, University of Maryland, College Park, MD, 20742, USA.
Weiye WangLaboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
Paul W KriebelLaboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.
Wolfgang LosertInstitute for Physical Science and Technology, Department of Physics, University of Maryland, College Park, MD, 20742, USA.
Daniel R LarsonLaboratory of Receptor Biology and Gene Expression, Center for Cancer Research, NCI, NIH, Bethesda, MD, 20892, USA.
Carole A ParentLaboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA. parentc@mail.nih.gov.ORCID 0000-0002-1518-1452
Center for Cancer Research · USUniversity of Maryland, College Park · US

Funding

Transcription Dynamics in Single CellsZIABC011383 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI LARSON, DANIEL · 2011 to 2025
$23.8M
Using Controlled 2D and 3D Nanotopography to Unravel Tactile Senses of Motile CelR01GM085574 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI FOURKAS, JOHN T, LOSERT, WOLFGANG · 2010 to 2014
$1.6M
NIGMS NIH HHS R01 GM085574
6 · The paper itself

Abstract

backgroundIn Dictyostelium discoideum, vesicular transport of the adenylyl cyclase A (ACA) to the posterior of polarized cells is essential to relay exogenous 3',5'-cyclic adenosine monophosphate (cAMP) signals during chemotaxis and for the collective migration of cells in head-to-tail arrangements called streams.

resultsUsing fluorescence in situ hybridization (FISH), we discovered that the ACA mRNA is asymmetrically distributed at the posterior of polarized cells. Using both standard estimators and Monte Carlo simulation methods, we found that the ACA mRNA enrichment depends on the position of the cell within a stream, with the posterior localization of ACA mRNA being strongest for cells at the end of a stream. By monitoring the recovery of ACA-YFP after cycloheximide (CHX) treatment, we observed that ACA mRNA and newly synthesized ACA-YFP first emerge as fluorescent punctae that later accumulate to the posterior of cells. We also found that the ACA mRNA localization requires 3' ACA cis-acting elements.

conclusionsTogether, our findings suggest that the asymmetric distribution of ACA mRNA allows the local translation and accumulation of ACA protein at the posterior of cells. These data represent a novel functional role for localized translation in the relay of chemotactic signal during chemotaxis.

Indexed as

Adenylyl CyclasesProtozoan ProteinsAnimalsCell PolarityCells, CulturedChemotaxisCycloheximideCytoplasmCytoplasmic StreamingDictyosteliumIn Situ Hybridization, FluorescenceProtein BiosynthesisRegulatory Sequences, Ribonucleic AcidRNA, MessengerRNA, ProtozoanRNA TransportAdenylyl CyclasesCycloheximideProtozoan ProteinsRegulatory Sequences, Ribonucleic AcidRNA, MessengerRNA, ProtozoanChemotaxisDictyosteliummRNASignal relay

Identifiers

PMID28545392
PMCPMC5445419
OpenAlexW2618318847

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.