ArticlePloS one2017
The role of the zinc finger protein ZC3H32 in bloodstream-form Trypanosoma brucei.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Life stage-specific poly(A) site selection regulated byProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Translational control byRNA (New York, N.Y.) · 2023Article
- Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes.Molecular & cellular proteomics : MCP · 2022Article
- Interactions of theParasitology · 2022Article
- Insights into the functions and RNA binding ofParasitology · 2021Article
- Article
- The ASCT/SCS cycle fuels mitochondrial ATP and acetate production in Trypanosoma brucei.Biochimica et biophysica acta. Bioenergetics · 2020Article
- Roles of the Pumilio domain protein PUF3 inParasitology · 2020Article
- The RNA-associated proteins MKT1 and MKT1L form alternative PBP1-containing complexes inThe Journal of biological chemistry · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.PLoS neglected tropical diseases · 2018Article
- The suppressive cap-binding complex factor 4EIP is required for normal differentiation.Nucleic acids research · 2018Article
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3 authors.
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Abstract
Kinetoplastids rely heavily on post-transcriptional mechanisms for control of gene expression, with regulation of mRNA processing, translation and degradation by RNA-binding proteins. ZC3H32 is a cytosolic mRNA-binding protein with three non-canonical CCCH zinc finger domains. It is much more abundant in bloodstream-form Trypanosoma brucei than in procyclic forms. Tethering of ZC3H32 to a reporter mRNA suppressed translation and resulted in mRNA degradation, and deletion analysis suggested that this activity was present in both the N- and C-terminal domains, but not the central zinc finger-containing domain. Tandem affinity purification, however, revealed no interaction partners that might account for this activity. RNASeq analyses did not yield any evidence for sequence-specific binding or regulation of specific mRNAs. The presence of ZC3H32 homologues in monogenetic and free-living Euglenids also argues against a role in developmental regulation, although its function may have diverged in evolution. T. brucei ZC3H32 might be implicated in basal mRNA metabolism, with this role perhaps being taken over by another protein in procyclic forms.
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