ArticleJournal of clinical laboratory analysis2018
High-resolution melting analysis assay for identification of Fonsecaea species.
Article in Journal of clinical laboratory analysis, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Article
- Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran.Current medical mycology · 2021Article
- [Long-pulsed 1064 nm Nd: YAG laser combined with terbinafine against chromoblastomycosis caused byNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Chromoblastomycosis Caused by Fonsecaea nubica: First Report in Northern China and Literature Review.Mycopathologia · 2019Review
- High-resolution melting analysis assay for identification of Fonsecaea species.Journal of clinical laboratory analysis · 2018Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChromoblastomycosis (CBM) is a chronic fungal disease. In China, the principle etiologic agent was a group of dematiaceous fungi, including Fonsecaea monophora, Fonsecaea nubica, and Cladophialophora carrionii. Although the Fonsecaea species have similar morphology, their pathogenicity is quite different. This study aims to establish a new solution for early identification of Fonsecaea species because of their distinctive potential infection risk.
methodsFive reference strains and 35 clinical isolates from patients with CBM, preserved in our laboratory, were used in this study. The universal primer ITS1 and ITS2 were chosen to amplify the highly conserved regions of rDNA. High-resolution melting (HRM) analysis was performed using the LIGHTCYCLER
resultsWe successfully differentiated the five strains according to their different Tm values and curve shapes. The 35 clinical isolates from patients were identified as 24 strains for F. monophora and 11 strains for F. nubica, which is consistent with the DNA sequencing results.
conclusionIt is the first time to use HRM analysis for identification of Fonsecaea species. Since the CBM etiologic agent in South China is mainly F. monophora and F. nubica, this strategy is sufficient to be applied in the clinical examination with high accuracy, speed, and throughput.
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