ArticlePloS one2017
TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- [Advances in immunomodulatory strategies for meniscal regeneration].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2026Review
- Enhanced lubricin secretion by human synovial mesenchymal stem cells compared to adipose mesenchymal stem cells.Stem cell research & therapy · 2025Article
- Integrin β1, PDGFRβ, and type II collagen are essential for meniscus regeneration by synovial mesenchymal stem cells in rats.Scientific reports · 2022Article
- BMP-4, TGF-β and Smad3 as Modulators of Synovial Fluid Cells Viability.Revista brasileira de ortopedia · 2022Article
- Synovial fluid niche promoted differentiation of dental follicle mesenchymal stem cells toward chondrogenesis in rheumatoid arthritis.Archives of rheumatology · 2022Article
- Synovial membrane mesenchymal stem cells for cartilaginous tissues repair.Molecular biology reports · 2022Review
- Recent advances to enhance the immunomodulatory potential of mesenchymal stem cells.Frontiers in immunology · 2022Review
- Regulating the fate of stem cells for regenerating the intervertebral disc degeneration.World journal of stem cells · 2021Review
- MSCs from polytrauma patients: preliminary comparative study with MSCs from elective-surgery patients.Stem cell research & therapy · 2021Article
- Thawed cryopreserved synovial mesenchymal stem cells show comparable effects to cultured cells in the inhibition of osteoarthritis progression in rats.Scientific reports · 2021Article
- Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators.World journal of stem cells · 2020Review
- Enhanced Chondrogenic Capacity of Mesenchymal Stem Cells After TNFα Pre-treatment.Frontiers in bioengineering and biotechnology · 2020Article
- Necrostatin-1 ameliorates the pathogenesis of experimental autoimmune encephalomyelitis by suppressing apoptosis and necroptosis of oligodendrocyte precursor cells.Experimental and therapeutic medicine · 2019Article
- Cryopreservation in 95% serum with 5% DMSO maintains colony formation and chondrogenic abilities in human synovial mesenchymal stem cells.BMC musculoskeletal disorders · 2019Article
- Time course analyses of structural changes in the infrapatellar fat pad and synovial membrane during inflammation-induced persistent pain development in rat knee joint.BMC musculoskeletal disorders · 2019Article
- Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic BehaviorStem cells international · 2019Article
- Repair mechanism of mesenchymal stem cells derived from nasal mucosa in orbital fracture.American journal of translational research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synovial mesenchymal stem cells (MSCs) are a candidate cell source for cartilage and meniscus regeneration. If we can proliferate synovial MSCs more effectively, we can expand clinical applications to patients with large cartilage and meniscus lesions. TNFα is a pleiotropic cytokine that can affect the growth and differentiation of cells in the body. The purpose of this study was to examine the effect of TNFα on proliferation, chondrogenesis, and other properties of human synovial MSCs. Passage 1 human synovial MSCs from 2 donors were cultured with 2.5 x 10-12~10-7 g/ml, 10 fold dilution series of TNFα for 14 days, then the cell number and colony number was counted. The effect of the optimum dose of TNFα on proliferation was also examined in synovial MSCs from 6 donors. Chondrogenic potential of synovial MSCs pretreated with TNFα was evaluated in 6 donors. The expressions of 12 surface antigens were also examined in 3 donors.2.5 ng/ml and higher concentration of TNFα significantly increased cell number/dish and cell number/colony in both donors. The effect of 25 ng/ml TNFα was confirmed in all 6 donors. There was no significant difference in the weight, or amount of glycosaminoglycan and DNA of the cartilage pellets between the MSCs untreated and MSCs pretreated with 25 ng/ml TNFα. TNFα decreased expression rate of CD 105 and 140b in all 3 donors. TNFα promoted proliferation of synovial MSCs with increase of cell number/ colony. Pretreatment with TNFα did not affect chondrogenesis of synovial MSCs. However, TNFα affected some properties of synovial MSCs.
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