ArticlePloS one2017
Metabolic flux analysis of heterotrophic growth in Chlamydomonas reinhardtii.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- An integrated biomass-to-algae pathway for producing emergency food components from lignocellulosic waste.Current research in food science · 2026Article
- Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism.Nature plants · 2025Article
- Eukaryotic viruses encode the ribosomal protein eL40.Npj viruses · 2024Article
- Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive.The Plant cell · 2023Article
- The way out: TPT3 allows triose-P export from the chloroplast.The Plant cell · 2023Article
- Accurate flux predictions using tissue-specific gene expression in plant metabolic modeling.Bioinformatics (Oxford, England) · 2023Article
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- Multi-Objective Optimization of Microalgae Metabolism: An Evolutive Algorithm Based on FBA.Metabolites · 2022Article
- Interactions Between Carbon Metabolism and Photosynthetic Electron Transport in aFrontiers in plant science · 2022Article
- Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte algaFrontiers in microbiology · 2022Article
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- Tracing metabolic flux through time and space with isotope labeling experiments.Current opinion in biotechnology · 2020Review
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- Cyanobacterial carboxysome mutant analysis reveals the influence of enzyme compartmentalization on cellular metabolism and metabolic network rigidity.Metabolic engineering · 2019Article
- Flux sampling is a powerful tool to study metabolism under changing environmental conditions.NPJ systems biology and applications · 2019Article
- The challenge and potential of photosynthesis: unique considerations for metabolic flux measurements in photosynthetic microorganisms.Biotechnology letters · 2019Review
- Modeling and Simulating the Aerobic Carbon Metabolism of a Green Microalga Using Petri Nets and New Concepts of VANESA.Journal of integrative bioinformatics · 2018Article
- Systems protobiology: origin of life in lipid catalytic networks.Journal of the Royal Society, Interface · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the wealth of knowledge available for C. reinhardtii, the central metabolic fluxes of growth on acetate have not yet been determined. In this study, 13C-metabolic flux analysis (13C-MFA) was used to determine and quantify the metabolic pathways of primary metabolism in C. reinhardtii cells grown under heterotrophic conditions with acetate as the sole carbon source. Isotopic labeling patterns of compartment specific biomass derived metabolites were used to calculate the fluxes. It was found that acetate is ligated with coenzyme A in the three subcellular compartments (cytosol, mitochondria and plastid) included in the model. Two citrate synthases were found to potentially be involved in acetyl-coA metabolism; one localized in the mitochondria and the other acting outside the mitochondria. Labeling patterns demonstrate that Acetyl-coA synthesized in the plastid is directly incorporated in synthesis of fatty acids. Despite having a complete TCA cycle in the mitochondria, it was also found that a majority of the malate flux is shuttled to the cytosol and plastid where it is converted to oxaloacetate providing reducing equivalents to these compartments. When compared to predictions by flux balance analysis, fluxes measured with 13C-MFA were found to be suboptimal with respect to biomass yield; C. reinhardtii sacrifices biomass yield to produce ATP and reducing equivalents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.