ArticlePloS one2017
A quantitative label-free analysis of the extracellular proteome of human supraspinatus tendon reveals damage to the pericellular and elastic fibre niches in torn and aged tissue.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Extracellular matrix composition of connective tissues: a systematic review and meta-analysis.Scientific reports · 2019Pooled it
- Application of Tendon-Derived Matrix and Carbodiimide Crosslinking Matures the Engineered Tendon-Like Proteome on Meltblown Scaffolds.Journal of tissue engineering and regenerative medicine · 2025Article
- Bioactive extracellular matrix fragments in tendon repair.Cell and tissue research · 2022Review
- Factor inhibiting HIF can catalyze two asparaginyl hydroxylations in VNVN motifs of ankyrin fold proteins.The Journal of biological chemistry · 2022Article
- Hierarchical ultrastructure: An overview of what is known about tendons and future perspective for tendon engineering.Bioactive materials · 2022Review
- Tendon and multiomics: advantages, advances, and opportunities.NPJ Regenerative medicine · 2021Review
- Peptide location fingerprinting reveals modification-associated biomarker candidates of ageing in human tissue proteomes.Aging cell · 2021Article
- Extracellular matrix at the muscle - tendon interface: functional roles, techniques to explore and implications for regenerative medicine.Connective tissue research · 2021Review
- Comparative Analysis of the Extracellular Matrix Proteome across the Myotendinous Junction.Journal of proteome research · 2020Article
- Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon.Scientific reports · 2020Article
- Spectrum of Tendon Pathologies: Triggers, Trails and End-State.International journal of molecular sciences · 2020Review
- The "other" 15-40%: The Role of Non-Collagenous Extracellular Matrix Proteins and Minor Collagens in Tendon.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- Combined ascorbic acid and TJournal of orthopaedic surgery and research · 2019Article
- Fatigue loading of tendon results in collagen kinking and denaturation but does not change local tissue mechanics.Journal of biomechanics · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tears of the human supraspinatus tendon are common and often cause painful and debilitating loss of function. Progressive failure of the tendon leading to structural abnormality and tearing is accompanied by numerous cellular and extra-cellular matrix (ECM) changes in the tendon tissue. This proteomics study aimed to compare torn and aged rotator cuff tissue to young and healthy tissue, and provide the first ECM inventory of human supraspinatus tendon generated using label-free quantitative LC-MS/MS. Employing two digestion protocols (trypsin and elastase), we analysed grain-sized tendon supraspinatus biopsies from older patients with torn tendons and from healthy, young controls. Our findings confirm measurable degradation of collagen fibrils and associated proteins in old and torn tendons, suggesting a significant loss of tissue organisation. A particularly marked reduction of cartilage oligomeric matrix protein (COMP) raises the possibility of using changes in levels of this glycoprotein as a marker of abnormal tissue, as previously suggested in horse models. Surprisingly, and despite using an elastase digestion for validation, elastin was not detected, suggesting that it is not highly abundant in human supraspinatus tendon as previously thought. Finally, we identified marked changes to the elastic fibre, fibrillin-rich niche and the pericellular matrix. Further investigation of these regions may yield other potential biomarkers and help to explain detrimental cellular processes associated with tendon ageing and tendinopathy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.