ArticlePloS one2017
Carbonyl-based blue autofluorescence of proteins and amino acids.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 95 citations in OpenAlex.
- Recent advances in the identification of vaginal fluid for forensic applications.International journal of legal medicine · 2026Review
- A Wavelength Rule for the Analysis of Clusteroluminescence.Polymers · 2025Article
- Anomalous Water Fluorescence Induced by Solutes.The journal of physical chemistry letters · 2025Article
- Crystallization of L-Cysteine in Heavy Water Induces Intrinsic Fluorescence.Angewandte Chemie (International ed. in English) · 2025Article
- Aβ-Aggregation-Generated Blue Autofluorescence Illuminates Senile Plaques as well as Complex Blood and Vascular Pathologies in Alzheimer's Disease.Neuroscience bulletin · 2024Article
- Photonics of Hydrothermally Treated β-Lactoglobulin Amyloids.Small science · 2024Article
- Self-Assembly of a Novel Pentapeptide into Hydrogelated Dendritic Architecture: Synthesis, Properties, Molecular Docking and Prospective Applications.Gels (Basel, Switzerland) · 2024Article
- The carbonyl-lock mechanism underlying non-aromatic fluorescence in biological matter.Nature communications · 2023Article
- Photoluminescence in mammal fur: 111 years of research.Journal of mammalogy · 2023Article
- A Comprehensive Analysis of the Intrinsic Visible Fluorescence Emitted by Peptide/Protein Amyloid-like Assemblies.International journal of molecular sciences · 2023Review
- Amyloid Fibrils of Stefin B Show Anisotropic Properties.International journal of molecular sciences · 2023Article
- Unveiling the Metal-Dependent Aggregation Properties of the C-terminal Region of Amyloidogenic Intrinsically Disordered Protein Isoforms DPF3b and DPF3a.International journal of molecular sciences · 2022Article
- One- and Two-Photon Excited Autofluorescence of Lysozyme Amyloids.The journal of physical chemistry letters · 2022Article
- Solid-state optical properties of self-assembling amyloid-like peptides with different charged states at the terminal ends.Scientific reports · 2022Article
- Fluorescence Emission of Self-assembling Amyloid-like Peptides: Solution versus Solid State.Chemphyschem : a European journal of chemical physics and physical chemistry · 2021Article
- On-off transition and ultrafast decay of amino acid luminescence driven by modulation of supramolecular packing.iScience · 2021Article
- Short hydrogen bonds enhance nonaromatic protein-related fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Two-Photon Excited Polarization-Dependent Autofluorescence of Amyloids as a Label-Free Method of Fibril Organization Imaging.The journal of physical chemistry letters · 2021Article
- Changes in Autofluorescence Level of Live and Dead Cells for Mouse Cell Lines.Journal of fluorescence · 2020Article
- Nucleation-dependent amyloid fibrillation of human GRASP55 in aqueous solution.European biophysics journal : EBJ · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Intrinsic protein fluorescence is inextricably linked to the near-UV autofluorescence of aromatic amino acids. Here we show that a novel deep-blue autofluorescence (dbAF), previously thought to emerge as a result of protein aggregation, is present at the level of monomeric proteins and even poly- and single amino acids. Just as its aggregation-related counterpart, this autofluorescence does not depend on aromatic residues, can be excited at the long wavelength edge of the UV and emits in the deep blue. Differences in dbAF excitation and emission peaks and intensities from proteins and single amino acids upon changes in solution conditions suggest dbAF's sensitivity to both the chemical identity and solution environment of amino acids. Autofluorescence comparable to dbAF is emitted by carbonyl-containing organic solvents, but not those lacking the carbonyl group. This implicates the carbonyl double bonds as the likely source for the autofluorescence in all these compounds. Using beta-lactoglobulin and proline, we have measured the molar extinction coefficients and quantum yields for dbAF in the monomeric state. To establish its potential utility in monitoring protein biophysics, we show that dbAF emission undergoes a red-shift comparable in magnitude to tryptophan upon thermal denaturation of lysozyme, and that it is sensitive to quenching by acrylamide. Carbonyl dbAF therefore provides a previously neglected intrinsic optical probe for investigating the structure and dynamics of amino acids, proteins and, by extension, DNA and RNA.
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