ArticleMolecular and cellular biology1988
Cell-dependent efficiency of reiterated nuclear signals in a mutant simian virus 40 oncoprotein targeted to the nucleus.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Multiple types of nuclear localization signals inBiochemistry and biophysics reports · 2024Article
- Recapitulation of the Function and Role of ROS Generated in Response to Heat Stress in Plants.Plants (Basel, Switzerland) · 2021Review
- All for One But Not One for All: Excitatory Synaptic Scaling and Intrinsic Excitability Are Coregulated by CaMKIV, Whereas Inhibitory Synaptic Scaling Is Under Independent Control.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Small Molecule Inhibitors Targeting Tec Kinase Block Unconventional Secretion of Fibroblast Growth Factor 2.The Journal of biological chemistry · 2016Article
- A versatile, bar-coded nuclear marker/reporter for live cell fluorescent and multiplexed high content imaging.PloS one · 2013Article
- Article
- Zinc finger transcription factor ecotropic viral integration site 1 is induced by all-trans retinoic acid (ATRA) and acts as a dual modulator of the ATRA response.The FEBS journal · 2009Article
- A versatile viral system for expression and depletion of proteins in mammalian cells.PloS one · 2009Article
- Novel role of antioxidant-1 (Atox1) as a copper-dependent transcription factor involved in cell proliferation.The Journal of biological chemistry · 2008Article
- Mechanical properties of the cell nucleus and the effect of emerin deficiency.Biophysical journal · 2006Article
- Hydrogen peroxide triggers nuclear export of telomerase reverse transcriptase via Src kinase family-dependent phosphorylation of tyrosine 707.Molecular and cellular biology · 2003Article
- Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.The Journal of cell biology · 1998Article
- An amino acid sequence motif sufficient for subnuclear localization of an arginine/serine-rich splicing factor.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- The inhibition of cultured myoblast differentiation by the simian virus 40 large T antigen occurs after myogenin expression and Rb up-regulation and is not exerted by transformation-competent cytoplasmic mutants.Journal of virology · 1995Article
- Nuclear transport of U1 snRNP in somatic cells: differences in signal requirement compared with Xenopus laevis oocytes.The Journal of cell biology · 1994Article
- Cooperation of proto-signals for nuclear accumulation of estrogen and progesterone receptors.The EMBO journal · 1992Article
- A hydrophobic protein sequence can override a nuclear localization signal independently of protein context.Molecular and cellular biology · 1991Article
- Analysis of the v-myb structural components important for transactivation of gene expression.Nucleic acids research · 1991Article
- Functional interaction of nuclear transport-defective simian virus 40 large T antigen with chromatin and nuclear matrix.Journal of virology · 1990Article
- Expression and targeting of intracellular antibodies in mammalian cells.The EMBO journal · 1990Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We investigated the requisites for, and functional consequences of, the relocation to the nucleus of a transforming nonkaryophilic mutant of the simian virus 40 large T antigen (a natural deletion mutant lacking an internal large-T-antigen domain that includes the signal for nuclear transport). Synthetic oligonucleotides were used to obtain gene variants with one or more copies of the signal-specifying sequence inserted near the gene 3' end, in a region dispensable for the main large-T-antigen functions. The analysis of stable transfectant populations showed that mouse NIH 3T3 cells, rat embryo fibroblasts, and simian CS cells (a subclone of CV1 cells) differed considerably in their ability to localize some variant molecules into the nucleus. CS cells were always the most efficient, and NIH 3T3 cells were the least efficient. The nuclear localization improved either with reiteration of the signal or with a left-flank modification of the signal amino acid context. Three signals appeared to be necessary and sufficient, even in NIH 3T3 cells, to obtain a nuclear accumulation comparable to that of wild-type simian virus 40 large T antigen; other signal-cell combinations caused a large variability in subcellular localization among cells of the same population, as if the nuclear uptake of some molecules depended on individual cell states. The effect of the modified location on the competence of the protein to alter cell growth was examined by comparing the activity of variants containing either the normal signal or a signal with a mutation (corresponding to large-T-antigen amino acid 128) that prevented nuclear transport. It was found that the nuclear variant was slightly more active than the cytoplasmic variants in rat embryo fibroblasts and NIH 3T3 cells and was notably less active in CS cells.
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