ArticleNucleic acids research2017
The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
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- Selecting genes for analysis using historically contingent progress: from RNA changes to protein-protein interactions.Nucleic acids research · 2025Article
- Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction.Journal of nanobiotechnology · 2024Article
- Selecting genes for analysis using historically contingent progress: from RNA changes to protein-protein interactions.bioRxiv : the preprint server for biology · 2024Article
- Article
- Target-specific requirements for RNA interference can arise through restricted RNA amplification despite the lack of specialized pathways.bioRxiv : the preprint server for biology · 2024Article
- Article
- Mating can initiate stable RNA silencing that overcomes epigenetic recovery.Nature communications · 2021Article
- Regulatory RNAs: A Universal Language for Inter-Domain Communication.International journal of molecular sciences · 2020Review
- New Strains for Tissue-Specific RNAi Studies inG3 (Bethesda, Md.) · 2020Article
- A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.Molecular cell · 2020Article
- Gene silencing by double-stranded RNA from C. elegans neurons reveals functional mosaicism of RNA interference.Nucleic acids research · 2019Article
- Construction of a germline-specific RNAi tool in C. elegans.Scientific reports · 2019Article
- Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.PLoS genetics · 2019Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
Long double-stranded RNA (dsRNA) can silence genes of matching sequence upon ingestion in many invertebrates and is therefore being developed as a pesticide. Such feeding RNA interference (RNAi) is best understood in the worm Caenorhabditis elegans, where the dsRNA-binding protein RDE-4 initiates silencing by recruiting an endonuclease to process long dsRNA into short dsRNA. These short dsRNAs are thought to move between cells because muscle-specific rescue of rde-4 using repetitive transgenes enables silencing in other tissues. Here, we extend this observation using additional promoters, report an inhibitory effect of repetitive transgenes, and discover conditions for cell-autonomous silencing in animals with tissue-specific rescue of rde-4. While expression of rde-4(+) in intestine, hypodermis, or neurons using a repetitive transgene can enable silencing also in unrescued tissues, silencing can be inhibited wihin tissues that express a repetitive transgene. Single-copy transgenes that express rde-4(+) in body-wall muscles or hypodermis, however, enable silencing selectively in the rescued tissue but not in other tissues. These results suggest that silencing by the movement of short dsRNA between cells is not an obligatory feature of feeding RNAi in C. elegans. We speculate that similar control of dsRNA movement could modulate tissue-specific silencing by feeding RNAi in other invertebrates.
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