Evidence map›Paper›PMID 28541550›Full record

ArticleInternational immunology2017

Integrity of immunoglobulin variable regions is supported by GANP during AID-induced somatic hypermutation in germinal center B cells.

Mohammed Mansour Abbas Eid, Mayuko Shimoda, Shailendra Kumar Singh, Sarah Ameen Almofty, Phuong Pham, Myron F Goodman, Kazuhiko Maeda, Nobuo Sakaguchi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. A high-resolution landscape of mutations in theProceedings of the National Academy of Sciences of the United States of America · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Mohammed Mansour Abbas EidClinical Pathology Department, Faculty of Medicine, Fayoum University, PO Box 63514, Fayoum, Egypt.
Mayuko ShimodaDepartment of Immunology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan.
Shailendra Kumar SinghLaboratory of Host Defense, World Premier International Research Center Initiative (WPI) Immunology Frontier Research Center (IFReC).
Sarah Ameen AlmoftyLaboratory of Immunology, Institute for Research and Medical Consultations (IRMC), University Of Dammam (UOD), PO Box 1982, Dammam 31441, Saudi Arabia.
Phuong PhamDepartments of Biological Sciences and Chemistry, University of Southern California, 1050 Childs Way, University Park, Los Angeles, CA 90089-2910, USA.
Myron F GoodmanDepartments of Biological Sciences and Chemistry, University of Southern California, 1050 Childs Way, University Park, Los Angeles, CA 90089-2910, USA.
Kazuhiko MaedaLaboratory of Host Defense, World Premier International Research Center Initiative (WPI) Immunology Frontier Research Center (IFReC).
Nobuo SakaguchiWorld Premier International Research Center Initiative (WPI) Immunology Frontier Research Center, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Osaka University · JPUniversity of Southern California · USFayoum University · EGTokyo Metropolitan Institute of Medical Science · JP

Funding

ERROR CORRECTION IN DNA SYNTHESIS: A BIOCHEMICAL STUDYR01GM021422 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI GOODMAN, MYRON · 1985 to 2014
$1.6M
Biochemical Basis of Somatic HypermutationR56ES013192 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI GOODMAN, MYRON · 2015 to 2015
$150k
NIEHS NIH HHS R56 ES013192NIGMS NIH HHS R01 GM021422
6 · The paper itself

Abstract

Immunoglobulin affinity maturation depends on somatic hypermutation (SHM) in immunoglobulin variable (IgV) regions initiated by activation-induced cytidine deaminase (AID). AID induces transition mutations by C→U deamination on both strands, causing C:G→T:A. Error-prone repairs of U by base excision and mismatch repairs (MMRs) create transversion mutations at C/G and mutations at A/T sites. In Neuberger's model, it remained to be clarified how transition/transversion repair is regulated. We investigate the role of AID-interacting GANP (germinal center-associated nuclear protein) in the IgV SHM profile. GANP enhances transition mutation of the non-transcribed strand G and reduces mutation at A, restricted to GYW of the AID hotspot motif. It reduces DNA polymerase η hotspot mutations associated with MMRs followed by uracil-DNA glycosylase. Mutation comparison between IgV complementary and framework regions (FWRs) by Bayesian statistical estimation demonstrates that GANP supports the preservation of IgV FWR genomic sequences. GANP works to maintain antibody structure by reducing drastic changes in the IgV FWR in affinity maturation.

Indexed as

AICDA (Activation-Induced Cytidine Deaminase)AnimalsAntibody AffinityBayes TheoremB-LymphocytesCells, CulturedCytidine DeaminaseDNA RepairGerminal CenterImmunoglobulin Variable RegionMiceMice, KnockoutMutationNuclear ProteinsPhosphoproteinsProtein ConformationAICDA (Activation-Induced Cytidine Deaminase)Cytidine DeaminaseGANP protein, mouseImmunoglobulin Variable RegionNuclear ProteinsPhosphoproteinsactivation-induced cytidine deaminaseaffinity maturationantibodyDNA repair pathwaysSHM

Identifiers

PMID28541550
PMCPMC5890899
OpenAlexW2618807073

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.