ArticlePlant cell reports2017
Phenotyping of VIGS-mediated gene silencing in rice using a vector derived from a DNA virus.
Article in Plant cell reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Functional validation of OsRPM1 as a positive regulator of bacterial blight resistance in rice via virus-induced gene silencing.Folia microbiologica · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- APathogens (Basel, Switzerland) · 2025Article
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Identification and functional validation of a new gene conferring resistance toFrontiers in plant science · 2024Article
- A Modified Method for Transient Transformation via Pollen Magnetofection inInternational journal of molecular sciences · 2023Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- A highly efficient genetic transformation system for broccoli and subcellular localization.Frontiers in plant science · 2023Article
- Knock-down of gene expression throughout meiosis and pollen formation by virus-induced gene silencing in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).Plant methods · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Rice aquaporin OsPIP2;2 is a water-transporting facilitator in relevance to drought-tolerant responses.Plant direct · 2021Article
- Simultaneous gene expression and multi-gene silencing in Zea mays using maize dwarf mosaic virus.BMC plant biology · 2021Article
- Dual Activities of Receptor-Like Kinase OsWAKL21.2 Induce Immune Responses.Plant physiology · 2020Article
- Hybrid Incompatibility of the Plant Immune System: An Opposite Force to Heterosis Equilibrating Hybrid Performances.Frontiers in plant science · 2020Review
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation.Journal of experimental botany · 2019Article
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Authors and funding
2 authors.
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Abstract
key messageTarget genes in rice can be optimally silenced if inserted in antisense or hairpin orientation in the RTBV-derived VIGS vector and plants grown at 28 °C and 80% humidity after inoculation. Virus induced gene silencing (VIGS) is a method used to transiently silence genes in dicot as well as monocot plants. For the important monocot species rice, the Rice tungro bacilliform virus (RTBV)-derived VIGS system (RTBV-VIGS), which uses agroinoculation to initiate silencing, has not been standardized for optimal use. Here, using RTBV-VIGS, three sets of conditions were tested to achieve optimal silencing of the rice marker gene phytoene desaturase (pds). The effect of orientation of the insert in the RTBV-VIGS plasmid (sense, antisense and hairpin) on the silencing of the target gene was then evaluated using rice magnesium chelatase subunit H (chlH). Finally, the rice Xa21 gene, conferring resistance against bacterial leaf blight disease (BLB) was silenced using RTBV-VIGS system. In each case, real-time PCR-based assessment indicated approximately 40-80% fall in the accumulation levels of the transcripts of pds, chlH and Xa21. In the case of pds, the appearance of white streaks in the emerging leaves, and for chlH, chlorophyll levels and F
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