ArticlePlant methods2017
A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in
Article in Plant methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 65 citations in OpenAlex.
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- ASCL1 promotes nuclear shrinkage in transdifferentiation by suppressing NUP37.Stem cell reports · 2026Article
- QTL mapping of vernalization-insensitive and photoperiod-independent early flowering in yellow lupin (Frontiers in plant science · 2026Article
- Mesophyll protoplasts: a key tool for deciphering plant nutrient starvation pathways.Frontiers in plant science · 2026Article
- TheInternational journal of molecular sciences · 2025Article
- Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton.Nature communications · 2025Article
- The receptor-like cytoplasmic kinase OsBSK1-2 regulates immunity via an HLH/bHLH complex.Journal of integrative plant biology · 2024Article
- Recent advances in exploring transcriptional regulatory landscape of crops.Frontiers in plant science · 2024Review
- Chromatin Immunoprecipitation Using Imbibed Seeds of Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Systematic evaluation of chromatin immunoprecipitation sequencing to study histone occupancy in dormancy transitions of grapevine buds.Tree physiology · 2023Article
- Thermo-Sensitive Spikelet Defects 1 acclimatizes rice spikelet initiation and development to high temperature.Plant physiology · 2023Article
- Approaches for Modes of Action Study of Long Non-Coding RNAs: From Single Verification to Genome-Wide Determination.International journal of molecular sciences · 2023Review
- Chromatin Immunoprecipitation (ChIP) to Study the Transcriptional Regulatory Network that Controls Iron Homeostasis in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Optimization of an Efficient Protoplast Transformation System for Transient Expression Analysis Using Leaves ofPlants (Basel, Switzerland) · 2022Article
- High temperature induces male sterility via MYB66-MYB4-Casein kinase I signaling in cotton.Plant physiology · 2022Article
- A DE1 BINDING FACTOR 1-GLABRA2 module regulates rhamnogalacturonan I biosynthesis in Arabidopsis seed coat mucilage.The Plant cell · 2022Article
- Antagonistic modules regulate photosynthesis-associated nuclear genes via GOLDEN2-LIKE transcription factors.Plant physiology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBinding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an indispensable tool used to investigate the binding of DNA-binding proteins (e.g., transcription factors) to their target sequences in vivo. ChIP assays require specific antibodies that recognize endogenous target transcription factors; however, in most cases, such specific antibodies are unavailable. To overcome this problem, many ChIP assays use transgenic plants that express epitope-tagged transcription factors and immunoprecipitate the protein with a tag-specific antibody. However, generating transgenic plants that stably express epitope-tagged proteins is difficult and time-consuming.
resultsHere, we present a rapid, efficient ChIP protocol using transient expression in
conclusionsThe rapid and simple nature of our ChIP assay using
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.