ArticleScientific reports2017
Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Metagenomics study to compare the taxonomic composition and metabolism of a lignocellulolytic microbial consortium cultured in different carbon conditions.World journal of microbiology & biotechnology · 2022Article
- Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels.Frontiers in microbiology · 2022Review
- Grazing Changed Plant Community Composition and Reduced Stochasticity of Soil Microbial Community Assembly of Alpine Grasslands on the Qinghai-Tibetan Plateau.Frontiers in plant science · 2022Article
- Succession and potential role of bacterial communities during Pleurotus ostreatus production.FEMS microbiology ecology · 2021Article
- Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review.Biotechnology for biofuels · 2021Review
- A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity fromProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh.Microbiome · 2021Article
- A microalgal-based preparation with synergistic cellulolytic and detoxifying action towards chemical-treated lignocellulose.Plant biotechnology journal · 2021Article
- Exploring the Lignin Catabolism Potential of Soil-Derived Lignocellulolytic Microbial Consortia by a Gene-Centric Metagenomic Approach.Microbial ecology · 2020Article
- Insights into microbial community structure and diversity in oil palm waste compost.3 Biotech · 2019Article
- Cellulase-Hemicellulase Activities and Bacterial Community Composition of Different Soils from Algerian Ecosystems.Microbial ecology · 2019Article
- Soil Microbial Networks Shift Across a High-Elevation Successional Gradient.Frontiers in microbiology · 2019Article
- Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans.Nature communications · 2018Article
- Microclimate exerts greater control over litter decomposition and enzyme activity than litter quality in an alpine forest-tundra ecotone.Scientific reports · 2018Article
- Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.Biotechnology for biofuels · 2018Article
- Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-β-xylanase activity.Biotechnology for biofuels · 2017Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing intracellular proteins that contaminate the metasecretome. Here we describe a technique for targeting the extracellular proteome, which was used to compare the metasecretome and meta-surface-proteome of two lignocellulose-degrading communities grown on wheat straw and rice straw. A combination of mass spectrometry-based proteomics coupled with metatranscriptomics enabled the identification of a unique secretome pool from these lignocellulose-degrading communities. This method enabled us to efficiently discriminate the extracellular proteins from the intracellular proteins by improving detection of actively secreted and transmembrane proteins. In addition to the expected carbohydrate active enzymes, our new method reveals a large number of unknown proteins, supporting the notion that there are major gaps in our understanding of how microbial communities degrade lignocellulosic substrates.
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