ArticleScientific reports2017
ABCC2 is associated with Bacillus thuringiensis Cry1Ac toxin oligomerization and membrane insertion in diamondback moth.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Downregulation of APN1 andApplied and environmental microbiology · 2024Article
- Structural changes upon membrane insertion of the insecticidal pore-forming toxins produced byFrontiers in insect science · 2023Review
- Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution.Genomics, proteomics & bioinformatics · 2022Article
- The crystal structure of Cry78Aa from Bacillus thuringiensis provides insights into its insecticidal activity.Communications biology · 2022Article
- Functional Diversity of the Lepidopteran ATP-Binding Cassette Transporters.Journal of molecular evolution · 2022Review
- Molecular and Kinetic Models for Pore Formation ofToxins · 2022Article
- Bacillus thuringiensis Cry1Ac Protoxin and Activated Toxin Exert Differential Toxicity Due to a Synergistic Interplay of Cadherin with ABCC Transporters in the Cotton Bollworm.Applied and environmental microbiology · 2022Article
- A versatile contribution of both aminopeptidases N and ABC transporters to Bt Cry1Ac toxicity in the diamondback moth.BMC biology · 2022Article
- A Novel Reference for Bt-Resistance Mechanism inInsects · 2021Article
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- The Essential and Enigmatic Role of ABC Transporters in Bt Resistance of Noctuids and Other Insect Pests of Agriculture.Insects · 2021Review
- Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.Frontiers in microbiology · 2021Review
- Independent and Synergistic Effects of Knocking out Two ABC Transporter Genes on Resistance toToxins · 2020Article
- Resistance to Bacillus thuringiensis Cry1Ac toxin requires mutations in two Plutella xylostella ATP-binding cassette transporter paralogs.PLoS pathogens · 2020Article
- The Cytocidal Spectrum ofToxins · 2020Review
- Study of theToxins · 2020Article
- Article
- TheToxins · 2019Article
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cry1A insecticidal toxins bind sequentially to different larval gut proteins facilitating oligomerization, membrane insertion and pore formation. Cry1Ac interaction with cadherin triggers oligomerization. However, a mutation in an ABC transporter gene (ABCC2) is linked to Cry1Ac resistance in Plutella xylostella. Cry1AcMod, engineered to lack helix α-1, was able to form oligomers without cadherinbinding and effectively countered Cry1Ac resistance linked to ABCC2. Here we analyzed Cry1Ac and Cry1AcMod binding and oligomerization by western blots using brush border membrane vesicles (BBMV) from a strain of P. xylostella susceptible to Cry1Ac (Geneva 88) and a strain with resistance to Cry1Ac (NO-QAGE) linked to an ABCC2 mutation. Resistance correlated with lack of specific binding and reduced oligomerization of Cry1Ac in BBMV from NO-QAGE. In contrast, Cry1AcMod bound specifically and still formed oligomers in BBMV from both strains. We compared association of pre-formed Cry1Ac oligomer, obtained by incubating Cry1Ac toxin with a Manduca sexta cadherin fragment, with BBMV from both strains. Our results show that pre-formed oligomers associate more efficiently with BBMV from Geneva 88 than with BBMV from NO-QAGE, indicating that the ABCC2 mutation also affects the association of Cry1Ac oligomer with the membrane. These data indicate, for the first time, that ABCC2 facilitates Cry1Ac oligomerization and oligomer membrane insertion in P. xylostella.
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