ArticleMolecular biology of the cell2017
Analysis of interphase node proteins in fission yeast by quantitative and superresolution fluorescence microscopy.
Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Eukaryotic cell size regulation and its implications for cellular function and dysfunction.Physiological reviews · 2024Review
- Processes Controlling the Contractile Ring during Cytokinesis in Fission Yeast, Including the Role of ESCRT Proteins.Journal of fungi (Basel, Switzerland) · 2024Review
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Article
- Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division.The Journal of cell biology · 2022Article
- The Development of Microscopic Imaging Technology and its Application in Micro- and Nanotechnology.Frontiers in chemistry · 2022Review
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- Sample Preparation and Imaging Conditions Affect mEos3.2 Photophysics in Fission Yeast Cells.Biophysical journal · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.Nano letters · 2020Article
- Article
- How the mechanobiome drives cell behavior, viewed through the lens of control theory.Journal of cell science · 2019Review
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry.eLife · 2019Article
- Fission yeast type 2 node proteins Blt1p and Gef2p cooperate to ensure timely completion of cytokinesis.BMC molecular and cell biology · 2019Article
- Reprogramming Cdr2-Dependent Geometry-Based Cell Size Control in Fission Yeast.Current biology : CB · 2019Article
- Cell size-dependent regulation of Wee1 localization by Cdr2 cortical nodes.The Journal of cell biology · 2018Article
- Controlling cell size through sizer mechanisms.Current opinion in systems biology · 2017Review
- The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells.Cell cycle (Georgetown, Tex.)Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We used quantitative confocal microscopy and FPALM superresolution microscopy of live fission yeast to investigate the structures and assembly of two types of interphase nodes-multiprotein complexes associated with the plasma membrane that merge together and mature into the precursors of the cytokinetic contractile ring. During the long G2 phase of the cell cycle, seven different interphase node proteins maintain constant concentrations as they accumulate in proportion to cell volume. During mitosis, the total numbers of type 1 node proteins (cell cycle kinases Cdr1p, Cdr2p, Wee1p, and anillin Mid1p) are constant even when the nodes disassemble. Quantitative measurements provide strong evidence that both types of nodes have defined sizes and numbers of constituent proteins, as observed for cytokinesis nodes. Type 1 nodes assemble in two phases-a burst at the end of mitosis, followed by steady increase during interphase to double the initial number. Type 2 nodes containing Blt1p, Rho-GEF Gef2p, and kinesin Klp8p remain intact throughout the cell cycle and are constituents of the contractile ring. They are released from the contractile ring as it disassembles and then associate with type 1 nodes around the equator of the cell during interphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.