ArticleBiophysical journal2017
A Simple Marker-Assisted 3D Nanometer Drift Correction Method for Superresolution Microscopy.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Automated Cyclic Super-Resolution Microscopy for Nanoscale Protein Mapping.bioRxiv : the preprint server for biology · 2026Article
- A Versatile Drift-Free Super-Resolution Imaging Method via Oblique Bright-Field Correlation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Super-resolution imaging of T lymphocyte activation reveals chromatin decondensation and disrupted nuclear envelope.Communications biology · 2024Article
- Toward drift-free high-throughput nanoscopy through adaptive intersection maximization.Science advances · 2024Article
- A new method to experimentally quantify dynamics of initial protein-protein interactions.Communications biology · 2024Article
- RegiSTORM: channel registration for multi-color stochastic optical reconstruction microscopy.BMC bioinformatics · 2023Article
- The Amyloid Precursor Protein Modulates the Position and Length of the Axon Initial Segment.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Particle Localization Using Local Gradients and Its Application to Nanometer Stabilization of a Microscope.ACS nano · 2022Article
- Changes in nuclear pore numbers control nuclear import and stress response of mouse hearts.Developmental cell · 2022Article
- Estimating the localization spread function of static single-molecule localization microscopy images.Biophysical journal · 2022Article
- Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe.Science advances · 2022Article
- Enhanced super-resolution microscopy by extreme value based emitter recovery.Scientific reports · 2021Article
- A mean shift algorithm for drift correction in localization microscopy.Biophysical reports · 2021Article
- Embedded nanometer position tracking based on enhanced phasor analysis.Optics letters · 2021Article
- Accurate localization microscopy by intrinsic aberration calibration.Nature communications · 2021Article
- 3D active stabilization for single-molecule imaging.Nature protocols · 2021Article
- Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.Current protocols in cytometry · 2020Article
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
- Super-resolution localization microscopy: Toward high throughput, high quality, and low cost.APL photonics · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
High-precision fluorescence microscopy such as superresolution imaging or single-particle tracking often requires an online drift correction method to maintain the stability of the three-dimensional (3D) position of the sample at a nanometer precision throughout the entire data acquisition process. Current online drift correction methods require modification of the existing two-dimensional (2D) fluorescence microscope with additional optics and detectors, which can be cumbersome and limit its use in many biological laboratories. Here we report a simple marker-assisted online drift correction method in which all 3D positions can be derived from fiducial markers on the coverslip of the sample on a standard 2D fluorescence microscope without additional optical components. We validate this method by tracking the long-term 3D stability of single-molecule localization microscopy at a precision of <2 and 5 nm in the lateral and axial dimension, respectively. We then provide three examples to evaluate the performance of the marker-assisted drift correction method. Finally, we give an example of a biological application of superresolution imaging of spatiotemporal alteration for a DNA replication structure with both low-abundance newly synthesized DNAs at the early onset of DNA synthesis and gradually condensed DNA structures during DNA replication. Using an isogenic breast cancer progression cell line model that recapitulates normal-like, precancerous, and tumorigenic stages, we characterize a distinction in the DNA replication process in normal, precancerous, and tumorigenic cells.
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