ArticleNature communications2017
A synthetic biochemistry platform for cell free production of monoterpenes from glucose.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
What it found
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Who cites it
75 citing papers in PubMed, 223 citations in OpenAlex.
- Timed batch inputs unlock substantially higher yields for enzymatic cascades.Nature chemistry · 2026Article
- De Novo Biosynthesis of Valinomycin From Glucose Using In Vitro Reconstituted Hybrid Pathways.Chembiochem : a European journal of chemical biology · 2026Article
- The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.Journal of fungi (Basel, Switzerland) · 2026Review
- Recent advances in biomass deconstruction, microbial conversion, artificial intelligence, and carbon capture for sustainable bioenergy.Bioresources and bioprocessing · 2026Review
- Targeted mutagenesis of a thermophilic mevalonate kinase for efficient catalysis at lower temperatures.Protein engineering, design & selection : PEDS · 2026Article
- Enhanced production of sabinene by engineered Saccharomyces cerevisiae from corn hydrolysates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Parameterization of cell-free systems with time-series data using KETCHUP.PLoS computational biology · 2025Article
- Thermostable Enzyme Variants in the Lower Mevalonate Pathway Improve Isoprenoid Production by Cell-Free Biocatalysis.ACS sustainable chemistry & engineering · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Biochemical synthesis of taxanes from mevalonate.Synthetic and systems biotechnology · 2024Article
- Cofactor recycling strategies for secondary metabolite production in cell-free protein expression systems.Biophysical reviews · 2024Review
- Efficient 2,3-Butanediol Production from Ethanol by a Modified Four-Enzyme Synthetic Biosystem.Molecules (Basel, Switzerland) · 2024Article
- A synthetic cell-free pathway for biocatalytic upgrading of one-carbon substrates.bioRxiv : the preprint server for biology · 2024Article
- Improving Aerobic Digestion of Food Waste by Adding a Personalized Microbial Inoculum.Current microbiology · 2024Article
- Review
- ATP-free in vitro biotransformation of starch-derived maltodextrin into poly-3-hydroxybutyrate via acetyl-CoA.Nature communications · 2024Article
- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- When synthetic biology meets medicine.Life medicine · 2024Review
- Article
- A Light-Driven In Vitro Enzymatic Biosystem for the Synthesis of α-Farnesene from Methanol.Biodesign research · 2024Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells. Here we design a system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes. Different monoterpenes are produced in our system by changing the terpene synthase enzyme. The system is stable for the production of limonene, pinene and sabinene, and can operate continuously for at least 5 days from a single addition of glucose. We obtain conversion yields >95% and titres >15 g l
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.