ArticleCellular & molecular biology letters2017
PPARγ agonist through the terminal differentiation phase is essential for adipogenic differentiation of fetal ovine preadipocytes.
Article in Cellular & molecular biology letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Molecular Genetics and Epigenetics Regulatory Role of miRNAs on Adipogenesis and Intramuscular Fat Development in Beef Cattle: In the Context of Meat Quality.Molecular biotechnology · 2026Review
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- Optimization of Adipogenic Differentiation Protocol for Murine and Human Cell Culture Models.Bio-protocol · 2026Article
- Lesson on obesity and anatomy of adipose tissue: new models of study in the era of clinical and translational research.Journal of translational medicine · 2024Review
- Acetate cellulose fibrous scaffold is suitable for cultivated fat production.Current research in food science · 2024Article
- Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species.International journal of molecular sciences · 2023Article
- Application of In Vitro Models for Studying the Mechanisms Underlying the Obesogenic Action of Endocrine-Disrupting Chemicals (EDCs) as Food Contaminants-A Review.International journal of molecular sciences · 2023Review
- Histological characteristics of hair follicles at different hair cycle andFrontiers in veterinary science · 2023Article
- An insight into brown/beige adipose tissue whitening, a metabolic complication of obesity with the multifactorial origin.Frontiers in endocrinology · 2023Review
- Large-Scale Expansion of Porcine Adipose-Derived Stem Cells Based on Microcarriers System for Cultured Meat Production.Foods (Basel, Switzerland) · 2022Article
- Sex-specific extracellular matrix remodeling during early adipogenic differentiation by gestational bisphenol A exposure.Chemosphere · 2022Article
- Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.Biomolecules · 2022Review
- Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells.Cellular & molecular biology letters · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- Brown and beige adipose tissue: a novel therapeutic strategy for obesity and type 2 diabetes mellitus.Adipocyte · 2021Review
- Article
- Automated lipid droplet quantification system for phenotypic analysis of adipocytes using CellProfiler.Toxicology mechanisms and methods · 2020Article
- Prospects and challenges for cell-cultured fat as a novel food ingredient.Trends in food science & technology · 2020Article
- PPARs as Nuclear Receptors for Nutrient and Energy Metabolism.Molecules (Basel, Switzerland) · 2019Review
- β4GalT1 Mediates PPARγ N-Glycosylation to Attenuate Microglia Inflammatory Activation.Inflammation · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough the 3T3-L1 preadipocyte cell line represents an informative model for
methodsFetal and adult ovine adipose and skin tissue harvest, preadipocyte and fibroblast isolation, proliferation, and standardization and optimization of a new adipogenic differentiation protocol. Use of commercial cell lines (3T3-L1 and NIH-3T3) for validation purposes. Oil red O stain and gene expression were used to validate adipogenic differentiation. ANOVA and Fisher's exact test were used to determine statistical significance.
resultsOur optimized adipogenic differentiation method included a prolonged adipogenic cocktail exposure time from 2 to 8 days, higher insulin concentration, and supplementation with the peroxisome proliferator-activated receptor gamma (PPARγ) agonist, rosiglitazone. This protocol was optimized for both, fetal and adult preadipocytes.
conclusionsOur protocol enables successful adipogenic differentiation of fetal and adult ovine preadipocytes. This work demonstrates that compared to the 3T3-L1 cell line, fetal ovine preadipocytes require a longer exposure to the differentiation cocktail, and the need for IMBX, dexamethasone, and/or the PPARγ agonist rosiglitazone through the terminal differentiation phase. They also require higher insulin concentration during differentiation to enhance lipid accumulation and similar to human primary preadipocytes, PPARγ agonist supplementation is also required for ovine adipogenic differentiation. This work highlights species-specific differences requirements for adipogenic differentiation and the need to develop standardized methods to investigate comparative adipocyte biology.
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