ArticleCellular & molecular biology letters2016
The Characteristics Of Human Bone-Derived Cells (HBDCS) during osteogenesis in vitro.
Article in Cellular & molecular biology letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 60 citations in OpenAlex.
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- Strontium doped bioglass incorporated hydrogel-based scaffold for amplified bone tissue regeneration.Scientific reports · 2022Article
- Docosahexaenoic acid improves altered mineralization proteins, the decreased quality of hydroxyapatite crystals and suppresses oxidative stress induced by high glucose.Experimental and therapeutic medicine · 2022Article
- MicroRNA-15a-5p plays a role in osteogenic MC3T3-E1 cells differentiation by targeting PDCD4 (programmed cell death 4) via Wnt/β-catenin dependent signaling pathway.Bioengineered · 2021Article
- Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants.NPJ Regenerative medicine · 2021Article
- Features of a simvastatin-loaded multi-layered co-electrospun barrier membrane for guided bone regeneration.Experimental and therapeutic medicine · 2021Article
- NELL-1 Increased the Osteogenic Differentiation and mRNA Expression of Spheroids Composed of Stem Cells.Medicina (Kaunas, Lithuania) · 2021Article
- Gradient bimetallic ion-based hydrogels for tissue microstructure reconstruction of tendon-to-bone insertion.Science advances · 2021Article
- Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study.Clinical oral investigations · 2021Article
- Human osteoclasts/osteoblasts 3D dynamic co‑culture system to study the beneficial effects of glucosamine on bone microenvironment.International journal of molecular medicine · 2021Article
- Reduced graphene oxide coating enhances osteogenic differentiation of human mesenchymal stem cells on Ti surfaces.Biomaterials research · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures.
methodsIn this study, we isolated human bone-derived cells (HBDCs) and promoted their differentiation into osteoblasts. The following parameters were evaluated: cell number and viability, total protein expression, alkaline phosphatase activity, collagenous matrix production and osteogenic genes expression, i.e., gene coding for type I collagen and alkaline phosphatase.
resultsIt was proved the results show that HBDCs intensively proliferate during the first 7 days of culture followed by differentiation accompanied by an increase in alkaline phosphatase activity. Moreover, it was observed that during the differentiation of HBDCs, the expression of integrin β1 increased.
conclusionsThe process was also accompanied by changes in cell shape and rearrangement of the actin cytoskeleton and focal contacts containing FAK and the integrin β1 subunit. We suggest that the β1 integrin subunit may be a suitable new target in studies of the differentiation of primary human osteoblasts in culture.
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