ArticleScientific reports2017
Generation and Characterization of Acid Tolerant Fibrobacter succinogenes S85.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- - Invited Review - Translational gut microbiome research for strategies to improve beef cattle production sustainability and meat quality.Animal bioscience · 2024Article
- Disorders of acid-base balance promote rumen lipopolysaccharide biosynthesis in dairy cows by modulating the microbiome.Frontiers in microbiology · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Stimulation of Bovicin HC5 Production and Selection of Improved Bacteriocin-Producing Streptococcus equinus HC5 Variants.Probiotics and antimicrobial proteins · 2021Article
- Influence of host genetics in shaping the rumen bacterial community in beef cattle.Scientific reports · 2020Article
- Sustainable Production of Microbial Isoprenoid Derived Advanced Biojet Fuels Using Different Generation Feedstocks: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Development and characterization of acidic-pH-tolerant mutants ofBiotechnology for biofuels · 2020Article
- Residual feed intake in beef cattle and its association with carcass traits, ruminal solid-fraction bacteria, and epithelium gene expression.Journal of animal science and biotechnology · 2018Article
- Article
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6 authors.
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Abstract
Microorganisms are key components for plant biomass breakdown within rumen environments. Fibrobacter succinogenes have been identified as being active and dominant cellulolytic members of the rumen. In this study, F. succinogenes type strain S85 was adapted for steady state growth in continuous culture at pH 5.75 and confirmed to grow in the range of pH 5.60-5.65, which is lower than has been reported previously. Wild type and acid tolerant strains digested corn stover with equal efficiency in batch culture at low pH. RNA-seq analysis revealed 268 and 829 genes were differentially expressed at pH 6.10 and 5.65 compared to pH 6.70, respectively. Resequencing analysis identified seven single nucleotide polymorphisms (SNPs) in the sufD, yidE, xylE, rlmM, mscL and dosC genes of acid tolerant strains. Due to the absence of a F. succinogenes genetic system, homologues in Escherichia coli were mutated and complemented and the resulting strains were assayed for acid survival. Complementation with wild-type or acid tolerant F. succinogenes sufD restored E. coli wild-type levels of acid tolerance, suggesting a possible role in acid homeostasis. Recent genetic engineering developments need to be adapted and applied in F. succinogenes to further our understanding of this bacterium.
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