ArticleMicrobial cell factories2017
Intracellular product recycling in high succinic acid producing yeast at low pH.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Influence of Different Fermentation Conditions on the Aroma-Active Compounds During New-Make Whisky Production Determined by GC-MS, GC×GC-O-MS, HPLC, and UPLC-MS.Molecules (Basel, Switzerland) · 2026Article
- Integrated Metabolomics and Proteomics Analysis of the Myocardium in a Mouse Model of Acute Viral Myocarditis.Immunity, inflammation and disease · 2025Article
- Succinic acid - A run-through of the latest perspectives of production from renewable biomass.Heliyon · 2024Review
- Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain.Biotechnology and bioengineering · 2021Article
- Alpha-ketoglutarate utilization in Saccharomyces cerevisiae: transport, compartmentation and catabolism.Scientific reports · 2020Article
- Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: Slow growth, low pH, and high COBiotechnology and bioengineering · 2020Article
- EngineeringFrontiers in bioengineering and biotechnology · 2020Article
- Metabolic switches from quiescence to growth in synchronized Saccharomyces cerevisiae.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Metabolomics analysis of gut barrier dysfunction in a trauma-hemorrhagic shock rat model.Bioscience reports · 2019Article
- Metabolic adjustment upon repetitive substrate perturbations using dynamicMicrobial cell factories · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, the product transport can become bidirectional, i.e. the acid is reentering the cell and is again exported or even catabolized. Here, a quantitative approach for the identification of product recycling fluxes is developed.
resultsThe metabolic flux distributions at two time-points of the fermentation process were analyzed.
conclusionsAdding labeled product without changing the extracellular environment allowed to quantify intracellular metabolic fluxes under high producing conditions and identify product degradation cycles. In the specific case of succinic acid production, compartmentation was found to play a major role, i.e. the presence of metabolic activity in two different cellular compartments lead to intracellular product degradation reducing the yield. We also observed that the flux from glucose to succinic acid branches at two points in metabolism: (1) At the level of pyruvate, and (2) at cytosolic malate which was not expected.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.