ArticleEpigenetics & chromatin2017
MeDIP-seq and nCpG analyses illuminate sexually dimorphic methylation of gonadal development genes with high historic methylation in turtle hatchlings with temperature-dependent sex determination.
Article in Epigenetics & chromatin, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- The transcriptomic signature of age and sex is not conserved in human primary myotubes.Skeletal muscle · 2026Article
- Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.Development (Cambridge, England) · 2025Article
- Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica).BMC genomics · 2024Article
- A Cautionary Tale of Sexing by Methylation: Hybrid Bisulfite-Conversion Sequencing of Immunoprecipitated Methylated DNA inAnimals : an open access journal from MDPI · 2022Article
- Cadmium Ecotoxic Effects on EmbryonicGenes · 2022Article
- Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish.International journal of molecular sciences · 2022Article
- Thermosensitive sex chromosome dosage compensation in ZZ/ZW softshell turtles,Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Developmental programming of DNA methylation and gene expression patterns is associated with extreme cardiovascular tolerance to anoxia in the common snapping turtle.Epigenetics & chromatin · 2021Article
- A brief review of vertebrate sex evolution with a pledge for integrative research: towards 'Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Brief exposure to warm temperatures reduces intron retention inBiology letters · 2021Article
- Identification of Histone Modifications Reveals a Role of H2b Monoubiquitination in Transcriptional Regulation ofInternational journal of biological sciences · 2021Article
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- Genome-wide DNA Methylation Analysis of Mantle Edge and Mantle Central from Pearl Oyster Pinctada fucata martensii.Marine biotechnology (New York, N.Y.) · 2020Article
- Review
- Embryonic Temperature Programs Phenotype in Reptiles.Frontiers in physiology · 2020Review
- Sexual dimorphism in brain transcriptomes of Amami spiny rats (Tokudaia osimensis): a rodent species where males lack the Y chromosome.BMC genomics · 2019Article
- Putative Independent Evolutionary Reversals from Genotypic to Temperature-Dependent Sex Determination are Associated with Accelerated Evolution of Sex-Determining Genes in Turtles.Journal of molecular evolution · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA methylation alters gene expression but not DNA sequence and mediates some cases of phenotypic plasticity. Temperature-dependent sex determination (TSD) epitomizes phenotypic plasticity where environmental temperature drives embryonic sexual fate, as occurs commonly in turtles. Importantly, the temperature-specific transcription of two genes underlying gonadal differentiation is known to be induced by differential methylation in TSD fish, turtle and alligator. Yet, how extensive is the link between DNA methylation and TSD remains unclear. Here we test for broad differences in genome-wide DNA methylation between male and female hatchling gonads of the TSD painted turtle
resultsTurtle global methylation was consistent with other vertebrates (57% of the genome, 78% of all CpG dinucleotides). Numerous genes predicted to regulate turtle gonadogenesis exhibited sex-specific methylation and were proximal to methylated repeats. nCpG distribution predicted actual turtle DNA methylation and was bimodal in gene promoters (as other vertebrates) and introns (unlike other vertebrates). Differentially methylated genes, including regulators of sexual development, had lower nCpG content indicative of higher historic methylation.
conclusionsOurs is the first evidence suggesting that sexually dimorphic DNA methylation is pervasive in turtle gonads (perhaps mediated by repeat methylation) and that it targets numerous regulators of gonadal development, consistent with the hypothesis that it may regulate thermosensitive transcription in TSD vertebrates. However, further research during embryogenesis will help test this hypothesis and the alternative that instead, most differential methylation observed in hatchlings is the by-product of sexual differentiation and not its cause.
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