ArticleProceedings of the National Academy of Sciences of the United States of America2017
Healthy offspring from freeze-dried mouse spermatozoa held on the International Space Station for 9 months.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 88 citations in OpenAlex.
- In vitro effects of green tea (Camellia sinensis) aqueous extract on sperm quality: Mitigating oxidative stress and sperm DNA fragmentation in freeze-dried human spermatozoa.Clinical and experimental reproductive medicine · 2026Article
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- Limitations of serial cloning in mammals.Nature communications · 2026Article
- A systematic review of microgravity on reproductive systems: Implications for space biology and human health.iScience · 2026Review
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A biological and ethical assessment of whether humans could or should reproduce in space.NPJ microgravity · 2025Review
- Germline transmission of cryopreserved mouse spermatogonial stem cells maintained on the International Space Station.Stem cell reports · 2025Article
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.Communications biology · 2025Article
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Motility of Mouse Spermatozoa Changes Differentially After 30-Minute Exposure Under Simulating Weightlessness and Hypergravity.International journal of molecular sciences · 2024Article
- Beyond Earth's bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space.Reproductive biology and endocrinology : RB&E · 2024Review
- Long-term space missions' effects on the human organism: what we do know and what requires further research.Frontiers in physiology · 2024Review
- Effect of microgravity on mammalian embryo development evaluated at the International Space Station.iScience · 2023Article
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Human development and reproduction in space-a European perspective.NPJ microgravity · 2023Review
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at -95 °C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls. Next-generation sequencing showed only minor genomic differences between offspring derived from space-preserved spermatozoa and controls, and all offspring grew to adulthood and had normal fertility. Thus, we demonstrate that although space radiation can damage sperm DNA, it does not affect the production of viable offspring after at least 9 mo of storage on the ISS.
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