Evidence map›Paper›PMID 28532672›Full record

ArticleToxicology and applied pharmacology2017

Molecular insights into the differences in anti-inflammatory activities of green tea catechins on IL-1β signaling in rheumatoid arthritis synovial fibroblasts.

Sabrina Fechtner, Anil Singh, Mukesh Chourasia, Salahuddin Ahmed

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology and applied pharmacology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed, 1 pooled it
6.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 1 synthesis or guideline pooled it, 111 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Sabrina FechtnerDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA, USA.
Anil SinghDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA, USA.
Mukesh ChourasiaDepartment of Pharmacoinformatics, National lnstitute of Pharmaceutical Education and Research, Hajipur, Bihar, India.
Salahuddin AhmedDepartment of Pharmaceutical Sciences, Washington State University College of Pharmacy, Spokane, WA, USA. Electronic address: salah.ahmed@wsu.edu.
Washington State University Spokane · USNational Institute of Pharmaceutical Education and Research · IN

Funding

Regulation of IL-6 mediated inflammation and tissue destruction by EGCGR01AR063104 · NIAMS · WASHINGTON STATE UNIVERSITY · PI AHMED, SALAH-UDDIN · 2013 to 2017
$1.7M
NIAMS NIH HHS R01 AR063104
6 · The paper itself

Abstract

In this study, we found that catechins found in green tea (EGCG, EGC, and EC) differentially interfere with the IL-1β signaling pathway which regulates the expression of pro-inflammatory mediators (IL-6 and IL-8) and Cox-2 in primary human rheumatoid arthritis synovial fibroblasts (RASFs). EGCG and EGC inhibited IL-6, IL-8, and MMP-2 production and selectively inhibited Cox-2 expression. EC did not exhibit any inhibitory effects. When we looked at the expression of key signaling proteins in the IL-1β signaling pathway, we found all the tested catechins could inhibit TAK-1 activity. Therefore, the consumption of green tea offers an overall anti-inflammatory effect. Molecular docking analysis confirms that EGCG, EGC, and EC all occupy the active site of the TAK1 kinase domain. However, EGCG occupies the majority of the TAK1 active site. In addition to TAK1 inhibition, EGCG can also inhibit P38 and nuclear NF-κB expression whereas EC and EGC were not effective inhibitors. Our findings suggest one of the main health benefits associated with the consumption of green tea are due to the activity of EGCG and EGC which are both present at higher amounts. Although EGCG is the most effective catechin at inhibiting downstream inflammatory signaling, its effectiveness could be hindered by the presence of EC. Therefore, varying EC content in green tea may reduce the anti-inflammatory effects of other potential catechins in green tea.

Indexed as

Anti-Inflammatory AgentsAntirheumatic AgentsArthritis, RheumatoidCatalytic DomainCatechinCells, CulturedCyclooxygenase 2 InhibitorsFibroblastsHumansInterleukin-1betaInterleukin-6Interleukin-8MAP Kinase Kinase Kinase 7MAP Kinase Kinase KinasesMatrix Metalloproteinase 2Molecular Docking SimulationAnti-Inflammatory AgentsAntirheumatic AgentsCatechinCXCL8 protein, humanCyclooxygenase 2 Inhibitorsepigallocatechin gallategallocatecholIL1B protein, humanIL6 protein, humanInterleukin-1betaInterleukin-6Interleukin-8MAP Kinase Kinase Kinase 7MAP Kinase Kinase KinasesMatrix Metalloproteinase 2MMP2 protein, humanNF-kappa Bp38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsProto-Oncogene Proteins c-junTeaCatechinsEGCEGCGGreen teaInflammationRheumatoid arthritis

Identifiers

PMID28532672
PMCPMC6686859
OpenAlexW2616508022

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.