ArticleMicrobial cell factories2017
Enhancing poly-γ-glutamic acid production in Bacillus amyloliquefaciens by introducing the glutamate synthesis features from Corynebacterium glutamicum.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 30 citations in OpenAlex.
- Coculture Corynebacterium glutamicum and Bacillus licheniformis for producing poly-γ-glutamic acid from glucose.Applied microbiology and biotechnology · 2025Article
- Stress response regulation to extracellular polymeric substances biosynthesis in Bacillus licheniformis.Microbial cell factories · 2025Article
- Poly (γ) glutamic acid: a unique microbial biopolymer with diverse commercial applicability.Frontiers in microbiology · 2024Review
- Modular Engineering to Enhance Keratinase Production for Biotransformation of Discarded Feathers.Applied biochemistry and biotechnology · 2023Article
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette ofSynthetic and systems biotechnology · 2022Article
- Metabolic Engineering of Central Carbon Metabolism of Bacillus licheniformis for Enhanced Production of Poly-γ-glutamic Acid.Applied biochemistry and biotechnology · 2021Article
- From Residues to Added-Value Bacterial Biopolymers as Nanomaterials for Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2021Review
- Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production.Microbial cell factories · 2020Article
- Characterization of a membrane enzymatic complex for heterologous production of poly-γ-glutamate inMetabolic engineering communications · 2020Article
- Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation.Biotechnology for biofuels · 2020Article
- Metabolic engineering of Bacillus amyloliquefaciens LL3 for enhanced poly-γ-glutamic acid synthesis.Microbial biotechnology · 2019Article
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPoly-γ-glutamic acid (γ-PGA) is a valuable polymer with glutamate as its sole precursor. Enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-PGA production, especially for those glutamate-independent γ-PGA producing strains. Corynebacterium glutamicum has long been used for industrial glutamate production and it exhibits some unique features for glutamate synthesis; therefore introduction of these metabolic characters into the γ-PGA producing strain might lead to increased intracellular glutamate availability, and thus ultimate γ-PGA production.
resultsIn this study, the unique glutamate synthesis features from C. glutamicum was introduced into the glutamate-independent γ-PGA producing Bacillus amyloliquefaciens NK-1 strain. After introducing the energy-saving NADPH-dependent glutamate dehydrogenase (NADPH-GDH) pathway, the NK-1 (pHT315-gdh) strain showed slightly increase (by 9.1%) in γ-PGA production. Moreover, an optimized metabolic toggle switch for controlling the expression of ɑ-oxoglutarate dehydrogenase complex (ODHC) was introduced into the NK-1 strain, because it was previously shown that the ODHC in C. glutamicum was completely inhibited when glutamate was actively produced. The obtained NK-PO1 (pHT01-xylR) strain showed 66.2% higher γ-PGA production than the NK-1 strain. However, the further combination of these two strategies (introducing both NADPH-GDH pathway and the metabolic toggle switch) did not lead to further increase of γ-PGA production but rather the resultant γ-PGA production was even lower than that in the NK-1 strain.
conclusionsWe proposed new metabolic engineering strategies to improve the γ-PGA production in B. amyloliquefaciens. The NK-1 (pHT315-gdh) strain with the introduction of NADPH-GDH pathway showed 9.1% improvement in γ-PGA production. The NK-PO1 (pHT01-xylR) strain with the introduction of a metabolic toggle switch for controlling the expression of ODHC showed 66.2% higher γ-PGA production than the NK-1 strain. This work proposed a new strategy for improving the target product in microbial cell factories.
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