ArticleThe journal of physical chemistry letters2017
Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation.
Article in The journal of physical chemistry letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites.Analytical chemistry · 2025Article
- Bright and photostable yellow fluorescent proteins for extended imaging.Nature communications · 2025Article
- Blue-shifted genetically encoded CaNeurophotonics · 2024Article
- Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging.Nature communications · 2023Article
- A blue-shifted genetically encoded CabioRxiv : the preprint server for biology · 2023Article
- Impact of the Protein Environment on Two-Photon Absorption Cross-Sections of the GFP Chromophore Anion Resolved at the XMCQDPT2 Level of Theory.International journal of molecular sciences · 2023Article
- Alternative Strategy for Spectral Tuning of Flavin-Binding Fluorescent Proteins.The journal of physical chemistry. B · 2023Article
- Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range.International journal of molecular sciences · 2022Article
- Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation.Cell reports methods · 2022Article
- Engineering rhodopsins' activation spectra using a FRET-based approach.Biophysical journal · 2022Article
- Functional Characterization of an In-Frame Deletion in the Basic Domain of the Retinal Transcription Factor ATOH7.International journal of molecular sciences · 2022Article
- A genetically encoded fluorescent biosensor for extracellular L-lactate.Nature communications · 2021Article
- NaNuTrap: a technique for in vivo cell nucleus labelling using nanobodies.Development (Cambridge, England) · 2021Article
- Rational engineering of ratiometric calcium sensors with bright green and red fluorescent proteins.Communications biology · 2021Article
- Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins.Frontiers in molecular biosciences · 2021Article
- High throughput instrument to screen fluorescent proteins under two-photon excitation.Biomedical optics express · 2020Article
- What is the Optimal Size of the Quantum Region in Embedding Calculations of Two-Photon Absorption Spectra of Fluorescent Proteins?Journal of chemical theory and computation · 2020Article
- Methoxy-Substituted Nitrodibenzofuran-Based Protecting Group with an Improved Two-Photon Action Cross-Section for Thiol Protection in Solid Phase Peptide Synthesis.The Journal of organic chemistry · 2020Article
- Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen.PloS one · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Fluorescent proteins (FPs) are indispensable markers for two-photon imaging of live tissue, especially in the brains of small model organisms. The quantity of physiologically relevant data collected, however, is limited by heat-induced damage of the tissue due to the high intensities of the excitation laser. We seek to minimize this damage by developing FPs with improved brightness. Among FPs with the same chromophore structure, the spectral properties can vary widely due to differences in the local protein environment. Using a physical model that describes the spectra of FPs containing the anionic green FP (GFP) chromophore, we predict that those that are blue-shifted in one-photon absorption will have stronger peak two-photon absorption cross sections. Following this prediction, we present 12 blue-shifted GFP homologues and demonstrate that they are up to 2.5 times brighter than the commonly used enhanced GFP (EGFP).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.