ArticleApplications in plant sciences2017
Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing.
Article in Applications in plant sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- The plastome sequence of Bactris gasipaes and evolutionary analysis in tribe Cocoseae (Arecaceae).PloS one · 2021Article
- Article
- Illumina sequencing of complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2020Article
- Methods of analysis of chloroplast genomes of CPlant methods · 2020Article
- Characterization of sequence variability hotspots in Cranichideae plastomes (Orchidaceae, Orchidoideae).PloS one · 2020Article
- Comparative Plastid Genomics of NeotropicalFrontiers in plant science · 2020Article
- Complete Chloroplast Genomes and Comparative Analysis of Sequences Evolution among SevenInternational journal of molecular sciences · 2019Article
- Comparative assessment shows the reliability of chloroplast genome assembly using RNA-seq.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premise of the studyHigh-throughput sequencing of genomic DNA can recover complete chloroplast genome sequences, but the sequence data are usually dominated by sequences from nuclear/mitochondrial genomes. To overcome this deficiency, a simple enrichment method for chloroplast DNA from small amounts of plant tissue was tested for eight plant species including a gymnosperm and various angiosperms.
methodsChloroplasts were enriched using a high-salt isolation buffer without any step gradient procedures, and enriched chloroplast DNA was sequenced by multiplexed high-throughput sequencing.
resultsUsing this simple method, significant enrichment of chloroplast DNA-derived reads was attained, allowing deep sequencing of chloroplast genomes. As an example, the chloroplast genome of the conifer DISCUSSION: This chloroplast enrichment method from small amounts of plant tissue will be particularly useful for studies that use sequencers with relatively small throughput and that cannot use large amounts of tissue (e.g., for endangered species).
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