Evidence map›Paper›PMID 28529766›Full record

ArticleCell discovery2017

Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation.

Miriam Landfors, Jostein Johansen, Jan Magnus Aronsen, Cathrine Broberg Vågbø, Louis C Doré, Chuan He, Ivar Sjaastad, Pål Sætrom, Péter Fedorcsák, John Arne Dahl and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell discovery, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Miriam LandforsDepartment of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Jostein JohansenDepartment of Cancer Research and Molecular Medicine, NTNU-Norwegian University of Science and Technology, Trondheim, Norway.
Jan Magnus AronsenInstitute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.
Cathrine Broberg VågbøDepartment of Cancer Research and Molecular Medicine, NTNU-Norwegian University of Science and Technology, Trondheim, Norway.
Louis C DoréDepartment of Chemistry, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
Chuan HeDepartment of Chemistry, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
Ivar SjaastadInstitute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.
Pål SætromDepartment of Cancer Research and Molecular Medicine, NTNU-Norwegian University of Science and Technology, Trondheim, Norway.
Péter FedorcsákDepartment of Reproductive Medicine, Oslo University Hospital, Oslo, Norway.
John Arne DahlDepartment of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Håvard AanesDepartment of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Markus FußerDepartment of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Arne KlunglandDepartment of Microbiology, Institute of Medical Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Oslo University Hospital · NONorwegian University of Science and Technology · NOHoward Hughes Medical Institute · USUniversity of Chicago · USUniversity of Oslo · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sertoli cells have dual roles during the cells' lifetime. In the juvenile mammal, Sertoli cells proliferate and create the structure of the testis, and during puberty they cease to proliferate and take on the adult role of supporting germ cells through spermatogenesis. Accordingly, many genes expressed in Sertoli cells during testis formation are repressed during spermatogenesis. 5-Hydroxymethylcytosine (5hmC) is a DNA modification enzymatically generated from 5mC and present in all investigated mammalian tissues at varying levels. Using mass spectrometry and immunofluorescence staining we identified a substantial Sertoli cell-specific global 5hmC increase during rat puberty. Chemical labeling, pull-down and sequencing of 5hmC-containing genomic DNA from juvenile and adult rat Sertoli cells revealed that genes that lose or gain 5hmC belong to different functional pathways and mirror the functions of the cells in the two different states. Loss of 5hmC is associated with genes involved in development and cell structure, whereas gain of 5hmC is associated with genes involved in cellular pathways pertaining to the function of the adult Sertoli cells. This redistribution during maturation shows that 5hmC is a dynamic nucleotide modification, correlated to gene expression.

Indexed as

5-hydroxymethylcytosinecellular maturationDNA modificationepigeneticsSertoli cells

Identifiers

PMID28529766
PMCPMC5423031
OpenAlexW2613413502

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.