ArticleEBioMedicine2017
High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay.
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Article in EBioMedicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
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Who cites it
37 citing papers in PubMed, 57 citations in OpenAlex.
- Tools to Quantify and Characterize the Persistent Reservoir in People with HIV-1: Focus on Non-B Subtypes.Viruses · 2026Review
- Monocytes across life span in HIV infection: lights and shadows.Current opinion in HIV and AIDS · 2025Review
- Digital PCR in Virology: Current Applications and Future Perspectives.Molecular diagnosis & therapy · 2025Review
- Specific quantification of inducible HIV-1 reservoir by RT-LAMP.Communications medicine · 2024Article
- Monocyte to macrophage differentiation and changes in cellular redox homeostasis promote cell type-specific HIV latency reactivation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A novel high-throughput microwell outgrowth assay for HIV-infected cells.Journal of virology · 2024Article
- Automated microarray platform for single-cell sorting and collection of lymphocytes following HIV reactivation.Bioengineering & translational medicine · 2023Article
- [Application of Droplet-Based Microfluidics in Microbial Research].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Recombinant Virus Quantification Using Single-Cell Droplet Digital PCR: A Method for Infectious Titer Quantification.Viruses · 2023Article
- Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level.Biophysical reports · 2022Article
- Recent Advances in Digital Biosensing Technology.Biosensors · 2022Review
- Insights Into Persistent HIV-1 Infection and Functional Cure: Novel Capabilities and Strategies.Frontiers in microbiology · 2022Review
- Single-Cell Sequencing Facilitates Elucidation of HIV Immunopathogenesis: A Review of Current Literature.Frontiers in immunology · 2022Review
- Ex Vivo Differentiation of Resting CD4+ T Lymphocytes Enhances Detection of Replication Competent HIV-1 in Viral Outgrowth Assays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Everolimus, an mTORC1/2 inhibitor, in ART-suppressed individuals who received solid organ transplantation: A prospective study.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2021Article
- Microfluidic devices for detection of RNA viruses.Reviews in medical virology · 2021Review
- Inducible HIV-1 Reservoir Quantification: Clinical Relevance, Applications and Advancements of TILDA.Frontiers in microbiology · 2021Article
- Microfluidics in Single-Cell Virology: Technologies and Applications.Trends in biotechnology · 2020Review
- A digital PCR system based on the thermal cycled chip with multi helix winding capillary.Scientific reports · 2020Article
- Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection.International journal of molecular medicine · 2020Article
Corrections and comments
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Authors and funding
21 authors at 6 institutions in 3 countries.
Robert W YuchaDivision of Infectious Diseases, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States.
Kristen S HobbsDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Emily HanhauserDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Louise E HoganDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Wildaliz NievesDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Mehmet O OzenBio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, CA 94304, United States.
Fatih InciBio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, CA 94304, United States.
Vanessa YorkDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Erica A GibsonDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Cassandra ThanhDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Hadi ShafieeDivision of Renal Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States; Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, United States.
Rami El AssalBio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, CA 94304, United States.
Maja KiselinovaHIV Translational Research Unit, Department of Internal Medicine, Ghent University and Ghent University Hospital, Ghent, Belgium.
Yvonne P RoblesDivision of Infectious Diseases, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States.
Helen BaeHarvard University, Faculty of Arts & Sciences, Cambridge, MA 02138, United States.
Kaitlyn S LeadabrandDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
ShuQi WangState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affliliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China; Institute for Translational Medicine, Zhejiang University, China.
Steven G DeeksPositive Health Program, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States.
Daniel R KuritzkesDivision of Infectious Diseases, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States; Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, United States.
Utkan DemirciBio-Acoustic MEMS in Medicine (BAMM) Laboratory, Canary Center at Stanford for Cancer Early Detection, Department of Radiology, Stanford School of Medicine, Palo Alto, CA 94304, United States. Electronic address: utkan@stanford.edu.
Timothy J HenrichDivision of Experimental Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110, United States. Electronic address: timothy.henrich@ucsf.edu.
University of California, San Francisco · USBrigham and Women's Hospital · USLemuel Shattuck Hospital · USGhent University Hospital · BEHarvard University · USState Key Laboratory of Diagnosis and Treatment of Infectious Diseases
Funding
Validation, CLIA and Qualification (VQC): Enhancing the RS ratio as a tool for AIDS Clinical Trial Group (ACTG) tuberculosis trialsUM1AI106701 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Grace M Aldrovandi · 2014 to 2026
$116.8MVirology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STEVEN Grant DEEKS · 1988 to 2026
$93.6MMOLECULAR AND CELLULAR IMMUNOLOGYT32AI007334 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CYSTER, JASON G · 1988 to 2023
$11.0MSingle Cell Characterization of Latent HIV-1 ReservoirsR01AI122862 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DEMIRCI, UTKAN, HENRICH, TIMOTHY JENSEN · 2016 to 2019
$1.5MImpact of Chemotherapy and Stem Cell Transplant on HIV-1 Reservoir DynamicsK23AI098480 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HENRICH, TIMOTHY JENSEN · 2012 to 2016
$673kA Novel Microfluidic HIV-1 Co-Culture Assay to Quantify Latent ReservoirsR21AI113117 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DEMIRCI, UTKAN, HENRICH, TIMOTHY JENSEN · 2014 to 2015
$480kMicrofluidic PCR Method to Identify and Characterize HIV-Infected Single CellsR21AI110277 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DEMIRCI, UTKAN, HENRICH, TIMOTHY JENSEN · 2014 to 2015
$474kNIAID NIH HHS K23 AI098480NIAID NIH HHS P30 AI027763NIAID NIH HHS R01 AI122862NIAID NIH HHS R21 AI110277NIAID NIH HHS R21 AI113117NIAID NIH HHS T32 AI007334NIAID NIH HHS UM1 AI106701
Abstract
Reactivation of latent viral reservoirs is on the forefront of HIV-1 eradication research. However, it is unknown if latency reversing agents (LRAs) increase the level of viral transcription from cells producing HIV RNA or harboring transcriptionally-inactive (latent) infection. We therefore developed a microfluidic single-cell-in-droplet (scd)PCR assay to directly measure the number of CD4
Indexed as
High-Throughput Screening AssaysPolymerase Chain ReactionRNA, ViralSingle-Cell AnalysisVirus LatencyAdultCD4-Positive T-LymphocytesCells, CulturedHIV-1HIV InfectionsHumansMiddle AgedSequence Analysis, DNAViral LoadVirus ActivationRNA, ViralDigital PCRHistone deacetylase inhibitorsHIV reactivationHIV reservoirsHuman Immunodeficiency Virus (HIV)Single cell quantification
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.