ArticlePlant reproduction2017
Dynamics of post-translationally modified histones during barley pollen embryogenesis in the presence or absence of the epi-drug trichostatin A.
Article in Plant reproduction, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat.Plants (Basel, Switzerland) · 2024Article
- Anther Culture Protocols for Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Roads to Haploid Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat.Frontiers in plant science · 2022Article
- Site-Directed Mutagenesis in Barley Using RNA-Guided Cas Endonucleases During Microspore-Derived Generation of Doubled Haploids.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Trichostatin A and sodium butyrate promotes plant regeneration in common wheat.Plant signaling & behavior · 2020Article
- Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route.Plants (Basel, Switzerland) · 2020Article
- Trichostatin A decreases the levels of MeCP2 expression and phosphorylation and increases its chromatin binding affinity.Epigenetics · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageImproving pollen embryogenesis. Despite the agro-economic importance of pollen embryogenesis, the mechanisms underlying this process are still poorly understood. We describe the dynamics of chromatin modifications (histones H3K4me2, H3K9ac, H3K9me2, and H3K27me3) and chromatin marks (RNA polymerase II CDC phospho-Ser5, and CENH3) during barley pollen embryogenesis. Immunolabeling results show that, in reaction to stress, immature pollen rapidly starts reorganizing several important chromatin modifications indicative of a change in cell fate. This new chromatin modification pattern was accomplished within 24 h from whereon it remained unaltered during subsequent mitotic activity. This indicates that cell fate transition, the central element of pollen embryogenesis, is completed early on during the induction process. Application of the histone deacetylase inhibitor trichostatin A stimulated pollen embryogenesis when used on pollen with a gametophytic style chromatin pattern. However, when this drug was administered to embryogenic pollen, the chromatin markers reversed toward a gametophytic profile, embryogenesis was halted and all pollen invariably died.
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