ArticleeLife2017
Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis.Communications biology · 2026Article
- Fluorescence Lifetime Microscopy Methods for Studying Dynamics of Fluorescent Proteins In Vivo and In Vitro.Advances in experimental medicine and biology · 2026Review
- Mitochondrial ROS trigger interorganellular signaling and prime ER processes to establish enhanced plant immunity.Science advances · 2025Article
- Phosphorylation of VAMP3 couples IL-6 exocytosis to dendritic cell activation.Journal of cell science · 2025Article
- Vamp3/syntaxin 4 mediates the basolateral membrane fusion of TfR transcytosis across the BBB and is exploited by pathogenicProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Single-sample image-fusion upsampling of fluorescence lifetime images.Science advances · 2024Article
- Transcriptomic response of lumpfish (Frontiers in immunology · 2024Article
- Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells.Cellular and molecular life sciences : CMLS · 2023Article
- Development and validation of a tumor immune cell infiltration-related gene signature for recurrence prediction by weighted gene co-expression network analysis in prostate cancer.Frontiers in genetics · 2023Article
- Fluorescence Lifetime Imaging of pH along the Secretory Pathway.ACS chemical biology · 2022Article
- Modulation of Immune Responses by Particle Size and Shape.Frontiers in immunology · 2020Review
- Interleukin-6 secretion is limited by self-signaling in endosomes.Journal of molecular cell biology · 2019Article
- Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules.FEBS letters · 2018Article
- Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy.Journal of visualized experiments : JoVE · 2017Article
- VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release.European journal of cell biology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SNARE proteins play a crucial role in intracellular trafficking by catalyzing membrane fusion, but assigning SNAREs to specific intracellular transport routes is challenging with current techniques. We developed a novel Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-based technique allowing visualization of real-time local interactions of fluorescently tagged SNARE proteins in live cells. We used FRET-FLIM to delineate the trafficking steps underlying the release of the inflammatory cytokine interleukin-6 (IL-6) from human blood-derived dendritic cells. We found that activation of dendritic cells by bacterial lipopolysaccharide leads to increased FRET of fluorescently labeled syntaxin 4 with VAMP3 specifically at the plasma membrane, indicating increased SNARE complex formation, whereas FRET with other tested SNAREs was unaltered. Our results revealed that SNARE complexing is a key regulatory step for cytokine production by immune cells and prove the applicability of FRET-FLIM for visualizing SNARE complexes in live cells with subcellular spatial resolution.
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