Evidence map›Paper›PMID 28523573›Full record

ArticleDigestive diseases and sciences2017

Activation of NLRP3 Inflammasome in Inflammatory Bowel Disease: Differences Between Crohn's Disease and Ulcerative Colitis.

Lazaros-Dimitrios Lazaridis, Aikaterini Pistiki, Evangelos J Giamarellos-Bourboulis, Marianna Georgitsi, Georgia Damoraki, Dimitrios Polymeros, George D Dimitriadis, Konstantinos Triantafyllou

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Digestive diseases and sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.

0numbers the graph read from it
0cells of the map it votes in
55citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

55 citing papers in PubMed, 100 citations in OpenAlex.

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  9. NLRP3 inflammasome inhibition decreasesInfection and immunity · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Lazaros-Dimitrios LazaridisHepatogastroenterology Unit, 2nd Department of Internal Medicine - Propaedeutic, Research Institute and Diabetes Center, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Aikaterini Pistiki4th Department of Internal Medicine, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Evangelos J Giamarellos-Bourboulis4th Department of Internal Medicine, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Marianna Georgitsi4th Department of Internal Medicine, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Georgia Damoraki4th Department of Internal Medicine, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Dimitrios PolymerosHepatogastroenterology Unit, 2nd Department of Internal Medicine - Propaedeutic, Research Institute and Diabetes Center, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
George D DimitriadisHepatogastroenterology Unit, 2nd Department of Internal Medicine - Propaedeutic, Research Institute and Diabetes Center, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece.
Konstantinos TriantafyllouHepatogastroenterology Unit, 2nd Department of Internal Medicine - Propaedeutic, Research Institute and Diabetes Center, Medical School, Attikon University General Hospital, National and Kapodistrian University, 1, Rimini Street, 124 62, Athens, Greece. ktriant@med.uoa.gr.ORCID 0000-0002-5183-9426
National and Kapodistrian University of Athens · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNLRP3 inflammasome is a multimolecular cytosol complex that, when activated, contributes to the cleavage of pro-interleukin (IL)-1β to IL-1β.

aimsTo investigate NLRP3 inflammasome activation in inflammatory bowel disease.

methodsPeripheral blood mononuclear cells from Crohn's disease (CD), ulcerative colitis (UC) patients and controls were stimulated with LPS in the absence or presence of MSU. After incubation, concentrations of IL-1β, IL-6, and TNFα were measured in cell supernatants and concentration of pro-IL-1β was measured in cell lysates. NLRP3 activation was defined as more than 30% increase in IL-1β production after MSU addition. In separate experiments, PBMCs were lysed for RNA isolation transcripts of IL-1β, TNFα, NLRP3, and CASP1 were measured by RT-PCR. DNA was isolated from CD patients for ATG16L1 gene genotyping.

resultsNLRP3 inflammasome was activated in 60% of CD patients compared to 28.6% of controls (p = 0.042); no significant difference was detected between UC and controls. Among UC patients, NLRP3 activation was associated (p = 0.008) with long-standing disease (>1.5 years). IL-1β levels were significantly higher in CD patents in comparison with controls (p = 0.032). No difference was detected in the levels of IL-6, TNFα, pro-IL-1β and in the numbers IL-1β, TNFα, NLRP3, and CASP1 transcripts among groups. IL-1β production was similar between carriers of wild-type and of SNP alleles of the rs2241880.

conclusionsNLRP3 inflammasome is activated in CD patients and in UC patients with long-standing disease.

Indexed as

AdultAgedColitis, UlcerativeCrohn DiseaseFemaleHumansInflammasomesInflammatory Bowel DiseasesLeukocytes, MononuclearMaleMiddle AgedNLR Family, Pyrin Domain-Containing 3 ProteinInflammasomesNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanATG16L1Crohn’s diseaseIBDNLRP3 inflammasomeUlcerative colitis

Identifiers

PMID28523573
OpenAlexW2616628260

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.