Evidence map›Paper›PMID 28523456›Full record

ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2017

Bone-marrow mesenchymal stem-cell administration significantly improves outcome after retinal ischemia in rats.

Biji Mathew, Jacqueline N Poston, John C Dreixler, Leianne Torres, Jasmine Lopez, Ruth Zelkha, Irina Balyasnikova, Maciej S Lesniak, Steven Roth

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Biji MathewDepartment of Anesthesiology, University of Illinois Medical Center, 1740 West Taylor Street, MC 515, Chicago, IL, 60612, USA.
Jacqueline N PostonDepartment of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA.
John C DreixlerDepartment of Anesthesia and Critical Care, University of Chicago, Chicago, IL, USA.
Leianne TorresDepartment of Anesthesiology, University of Illinois Medical Center, 1740 West Taylor Street, MC 515, Chicago, IL, 60612, USA.
Jasmine LopezDepartment of Anesthesiology, University of Illinois Medical Center, 1740 West Taylor Street, MC 515, Chicago, IL, 60612, USA.
Ruth ZelkhaDepartment of Ophthalmology, University of Illinois at Chicago, Chicago, IL, USA.
Irina BalyasnikovaDepartment of Neurosurgery, Northwestern University, Chicago, IL, USA.
Maciej S LesniakDepartment of Neurosurgery, Northwestern University, Chicago, IL, USA.
Steven RothDepartment of Anesthesiology, University of Illinois Medical Center, 1740 West Taylor Street, MC 515, Chicago, IL, 60612, USA. rothgas@uic.edu.ORCID http://orcid.org/0000-0002-4955-9855
University of Illinois Chicago · USNorthwestern University · USUniversity of Chicago · US

Funding

TRANSLATIONAL RESEARCH UNIVERSITY OF CHICAGO: CLINICAL TRIALSUL1RR024999 · NCRR · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2007 to 2011
$25.3M
Re-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2M
Translational Core for Therapeutic and Diagnostic DevelopmentP30EY001792 · NEI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI AAKALU, VINAY · 1985 to 2025
$14.8M
The Role of Adenosine in Retinal IschemiaR01EY010343 · NEI · UNIVERSITY OF CHICAGO · PI ROTH, STEVEN · 1994 to 2014
$4.8M
Intranasal Stem-Cell Based Therapy for GlioblastomaR01NS087990 · NINDS · UNIVERSITY OF CHICAGO · PI BALYASNIKOVA, IRINA V · 2014 to 2018
$2.1M
Short Term Aging-Related Research ProgramT35AG029795 · NIA · UNIVERSITY OF CHICAGO · PI Vineet Arora, DAVID O MELTZER · 2008 to 2026
$1.4M
NCATS NIH HHS UL1 TR000430NCRR NIH HHS UL1 RR024999NEI NIH HHS P30 EY001792NEI NIH HHS R01 EY010343NIA NIH HHS T35 AG029795NINDS NIH HHS R01 NS087990
6 · The paper itself

Abstract

purposeIschemia-associated retinal degeneration is one of the leading causes of vision loss, and to date, there are no effective treatment options. We hypothesized that delayed injection of bone-marrow stem cells (BMSCs) 24 h after the onset of ischemia could effectively rescue ischemic retina from its consequences, including apoptosis, inflammation, and increased vascular permeability, thereby preventing retinal cell loss.

methodsRetinal ischemia was induced in adult Wistar rats by increasing intraocular pressure (IOP) to 130-135 mmHg for 55 min. BMSCs harvested from rat femur were injected into the vitreous 24 h post-ischemia. Functional recovery was assessed 7 days later using electroretinography (ERG) measurements of the a-wave, b-wave, P2, scotopic threshold response (STR), and oscillatory potentials (OP). The retinal injury and anti-ischemic effects of BMSCs were quantitated by measuring apoptosis, autophagy, inflammatory markers, and retinal-blood barrier permeability. The distribution and fate of BMSC were qualitatively examined using real-time fundus imaging, and retinal flat mounts.

resultsIntravitreal delivery of BMSCs significantly improved recovery of the ERG a- and b-waves, OP, negative STR, and P2, and attenuated apoptosis as evidenced by decreased TUNEL and caspase-3 protein levels. BMSCs significantly increased autophagy, decreased inflammatory mediators (TNF-α, IL-1β, IL-6), and diminished retinal vascular permeability. BMSCs persisted in the vitreous and were also found within ischemic retina.

conclusionsTaken together, our results indicate that intravitreal injection of BMSCs rescued the retina from ischemic damage in a rat model. The mechanisms include suppression of apoptosis, attenuation of inflammation and vascular permeability, and preservation of autophagy.

Indexed as

AnimalsApoptosisBlood-Retinal BarrierBone Marrow CellsCapillary PermeabilityDisease Models, AnimalElectroretinographyIn Situ Nick-End LabelingIntravitreal InjectionsIschemiaMaleMesenchymal Stem Cell TransplantationRatsRats, WistarRetinal DegenerationRetinal VesselsApoptosisAutophagyBone-marrow mesenchymal stem cellsElectroretinographyInflammationIschemiaRetinal vascular permeability

Identifiers

PMID28523456
PMCPMC5841582
OpenAlexW2616764160

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.