ArticleNeurophotonics2017
Imaging membrane potential changes from dendritic spines using computer-generated holography.
Article in Neurophotonics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.Journal of biophotonics · 2025Article
- Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging.Scientific reports · 2024Article
- Electrophysiology of Dendritic Spines: Information Processing, Dynamic Compartmentalization, and Synaptic Plasticity.Advances in neurobiology · 2023Article
- Photoactivated voltage imaging in tissue with an archaerhodopsin-derived reporter.Science advances · 2021Article
- Voltage Imaging with a NIR-Absorbing Phosphine Oxide Rhodamine Voltage Reporter.Journal of the American Chemical Society · 2021Article
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning.Cell reports · 2020Article
- Imaging Subthreshold Voltage Oscillation With Cellular Resolution in the Inferior OliveFrontiers in cellular neuroscience · 2020Article
- 3D Localization for Light-Field Microscopy via Convolutional Sparse Coding on Epipolar Images.IEEE transactions on computational imaging · 2020Article
- Depth-resolved fiber photometry with a single tapered optical fiber implant.Nature methods · 2019Article
- A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation.PloS one · 2019Article
- Review
- High speed functional imaging with source localized multifocal two-photon microscopy.Biomedical optics express · 2018Article
- Improvements in Simultaneous Sodium and Calcium Imaging.Frontiers in cellular neuroscience · 2018Article
- Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits.Frontiers in cellular neuroscience · 2018Review
- Vortex-free phase profiles for uniform patterning with computer-generated holography.Optics express · 2017Article
- Recording Spikes Activity in Cultured Hippocampal Neurons Using Flexible or Transparent Graphene Transistors.Frontiers in neuroscience · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Electrical properties of neuronal processes are extraordinarily complex, dynamic, and, in the general case, impossible to predict in the absence of detailed measurements. To obtain such a measurement one would, ideally, like to be able to monitor electrical subthreshold events as they travel from synapses on distal dendrites and summate at particular locations to initiate action potentials. It is now possible to carry out these measurements at the scale of individual dendritic spines using voltage imaging. In these measurements, the voltage-sensitive probes can be thought of as transmembrane voltmeters with a linear scale, which directly monitor electrical signals. Grinvald et al. were important early contributors to the methodology of voltage imaging, and they pioneered some of its significant results. We combined voltage imaging and glutamate uncaging using computer-generated holography. The results demonstrated that patterned illumination, by reducing the surface area of illuminated membrane, reduces photodynamic damage. Additionally, region-specific illumination practically eliminated the contamination of optical signals from individual spines by the scattered light from the parent dendrite. Finally, patterned illumination allowed one-photon uncaging of glutamate on multiple spines to be carried out in parallel with voltage imaging from the parent dendrite and neighboring spines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.