Evidence map›Paper›PMID 28522808›Full record

ArticleScientific reports2017

Plasmonics Enhanced Smartphone Fluorescence Microscopy.

Qingshan Wei, Guillermo Acuna, Seungkyeum Kim, Carolin Vietz, Derek Tseng, Jongjae Chae, Daniel Shir, Wei Luo, Philip Tinnefeld, Aydogan Ozcan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Article
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  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. DNA Origami-Enabled Plasmonic Sensing.The journal of physical chemistry. C, Nanomaterials and interfaces · 2021
    Review
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Qingshan WeiElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Guillermo AcunaInstitute for Physical & Theoretical Chemistry, Braunschweig University of Technology, Braunschweig, 38106, Germany.
Seungkyeum KimChemical and Biomolecular Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Carolin VietzInstitute for Physical & Theoretical Chemistry, Braunschweig University of Technology, Braunschweig, 38106, Germany.
Derek TsengElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jongjae ChaeElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID 0000-0002-6560-197X
Daniel ShirElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Wei LuoElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Philip TinnefeldInstitute for Physical & Theoretical Chemistry, Braunschweig University of Technology, Braunschweig, 38106, Germany. p.tinnefeld@tu-braunschweig.de.
Aydogan OzcanElectrical Engineering Department, University of California, Los Angeles, Los Angeles, CA, 90095, USA. ozcan@ucla.edu.ORCID 0000-0002-0717-683X

Funding

ARRA NIH HHSHoward Hughes Medical Institute
6 · The paper itself

Abstract

Smartphone fluorescence microscopy has various applications in point-of-care (POC) testing and diagnostics, ranging from e.g., quantification of immunoassays, detection of microorganisms, to sensing of viruses. An important need in smartphone-based microscopy and sensing techniques is to improve the detection sensitivity to enable quantification of extremely low concentrations of target molecules. Here, we demonstrate a general strategy to enhance the detection sensitivity of a smartphone-based fluorescence microscope by using surface-enhanced fluorescence (SEF) created by a thin metal-film. In this plasmonic design, the samples are placed on a silver-coated glass slide with a thin spacer, and excited by a laser-diode from the backside through a glass hemisphere, generating surface plasmon polaritons. We optimized this mobile SEF system by tuning the metal-film thickness, spacer distance, excitation angle and polarization, and achieved ~10-fold enhancement in fluorescence intensity compared to a bare glass substrate, which enabled us to image single fluorescent particles as small as 50 nm in diameter and single quantum-dots. Furthermore, we quantified the detection limit of this platform by using DNA origami-based brightness standards, demonstrating that ~80 fluorophores per diffraction-limited spot can be readily detected by our mobile microscope, which opens up new opportunities for POC diagnostics and sensing applications in resource-limited-settings.

Indexed as

SmartphoneGlassImage EnhancementMicroscopy, FluorescencePoint-of-Care TestingQuantum DotsSilverSilver

Identifiers

PMID28522808
PMCPMC5437072

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.