ArticleGenome biology2017
The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana.
Article in Genome biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.
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Who cites it
71 citing papers in PubMed, 1 synthesis or guideline pooled it, 166 citations in OpenAlex.
- Overview of molecular mechanisms of plant leaf development: a systematic review.Frontiers in plant science · 2023Pooled it
- Histone variant H2A.X represses deposition of active H3K4me3 marks and restricts H2A.W incorporation.Nucleic acids research · 2026Article
- Comparative characterization of chromatin-targeting mechanisms across seven H3K4 methyltransferases in Arabidopsis.Plant communications · 2026Article
- Chromatin-associated DEK proteins maintain H3K27me3 balance and coordinate developmental transitions in plants.The New phytologist · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- DDM1 Maintains Heterochromatin by Regulating Histone Variants.International journal of molecular sciences · 2025Review
- Single amino acid mutations in histone H3.3 illuminate the functional significance of H3K4 methylation in plants.Nature communications · 2025Article
- A semidominant point mutation of Mediator tail subunit MED5b in Arabidopsis leads to altered enrichment of H3K27me3 and reduced expression of targets of MYC2.G3 (Bethesda, Md.) · 2025Article
- Heterochromatin in plant meiosis.Nucleus (Austin, Tex.) · 2024Review
- H2A.Z removal mediates the activation of genes accounting for cell elongation under light and temperature stress.Plant cell reports · 2024Review
- Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.Nature communications · 2024Article
- The Histone Variant H3.3 Is Required for Plant Growth and Fertility inInternational journal of molecular sciences · 2024Article
- Mechanisms of Plant Epigenetic Regulation in Response to Plant Stress: Recent Discoveries and Implications.Plants (Basel, Switzerland) · 2024Review
- PIF transcription factors-versatile plant epigenome landscapers.Frontiers in epigenetics and epigenomics · 2024Review
- Review
- The chromatin source-sink hypothesis: a shared mode of chromatin-mediated regulations.Development (Cambridge, England) · 2023Article
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- Chromatin remodeling of histone H3 variants underlies epigenetic inheritance of DNA methylation.bioRxiv : the preprint server for biology · 2023Article
- The master growth regulator DELLA binding to histone H2A is essential for DELLA-mediated global transcription regulation.Nature plants · 2023Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 4 institutions in 5 countries.
Funding
Abstract
backgroundGene bodies of vertebrates and flowering plants are occupied by the histone variant H3.3 and DNA methylation. The origin and significance of these profiles remain largely unknown. DNA methylation and H3.3 enrichment profiles over gene bodies are correlated and both have a similar dependence on gene transcription levels. This suggests a mechanistic link between H3.3 and gene body methylation.
resultsWe engineered an H3.3 knockdown in Arabidopsis thaliana and observed transcription reduction that predominantly affects genes responsive to environmental cues. When H3.3 levels are reduced, gene bodies show a loss of DNA methylation correlated with transcription levels. To study the origin of changes in DNA methylation profiles when H3.3 levels are reduced, we examined genome-wide distributions of several histone H3 marks, H2A.Z, and linker histone H1. We report that in the absence of H3.3, H1 distribution increases in gene bodies in a transcription-dependent manner.
conclusionsWe propose that H3.3 prevents recruitment of H1, inhibiting H1's promotion of chromatin folding that restricts access to DNA methyltransferases responsible for gene body methylation. Thus, gene body methylation is likely shaped by H3.3 dynamics in conjunction with transcriptional activity.
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