ArticleNucleic acids research2017
Chemical ligation of oligonucleotides using an electrophilic phosphorothioester.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Non-enzymatic conversion of RNA sequence information into DNA by squaramide ligation for accurate RNA quantification.Communications chemistry · 2026Article
- Analysis of the Effects of Sugar Modifications on RNA Chemical Ligation Reactions.Chembiochem : a European journal of chemical biology · 2025Article
- From liquid-phase synthesis to chemical ligation: preparation of oligonucleotides and their backbone analogs in solution.Nucleic acids research · 2025Review
- Chemical autoligation with phosphorothioate- and sulfonamide-terminated DNA via intramolecular cross-activation.Communications chemistry · 2025Article
- Artificial nucleic acid backbones and their applications in therapeutics, synthetic biology and biotechnology.Emerging topics in life sciences · 2021Article
- Engineering nucleosomes for generating diverse chromatin assemblies.Nucleic acids research · 2021Article
- Squaramides and Ureas: A Flexible Approach to Polymerase-Compatible Nucleic Acid Assembly.Angewandte Chemie (International ed. in English) · 2020Article
- HPLC methods for purity evaluation of man-made single-stranded RNAs.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a new approach for chemical ligation of oligonucleotides using the electrophilic phosphorothioester (EPT) group. A nucleophilic phosphorothioate group on oligonucleotides was converted into the EPT group by treatment with Sanger's reagent (1-fluoro-2,4-dinitrobenzene). EPT oligonucleotides can be isolated, stored frozen, and used for the ligation reaction. The reaction of the EPT oligonucleotide and an amino-modified oligonucleotide took place without any extra reagents at pH 7.0-8.0 at room temperature, and resulted in a ligation product with a phosphoramidate bond with a 39-85% yield. This method has potential uses in biotechnology and chemical biology.
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Registered trials
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