ArticleNucleic acids research2017
H2B ubiquitylation and the histone chaperone Asf1 cooperatively mediate the formation and maintenance of heterochromatin silencing.
Article in Nucleic acids research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Trypanosomatid histones: the building blocks of the epigenetic code of highly divergent eukaryotes.The Biochemical journal · 2025Review
- Histone chaperone HIRA complex regulates retrotransposons in embryonic stem cells.Stem cell research & therapy · 2022Article
- Redox Regulation of the NOR Transcription Factor Is Involved in the Regulation of Fruit Ripening in Tomato.Plant physiology · 2020Article
- Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions.Nucleic acids research · 2020Article
- TheeLife · 2019Article
- The interplay of histone H2B ubiquitination with budding and fission yeast heterochromatin.Current genetics · 2018Review
- The evolutionarily conserved factor Sus1/ENY2 plays a role in telomere length maintenance.Current genetics · 2018Article
- Recruitment and allosteric stimulation of a histone-deubiquitinating enzyme during heterochromatin assembly.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin is a heritable form of gene repression, with critical roles in development and cell identity. Understanding how chromatin factors results in such repression is a fundamental question. Chromatin is assembled and disassembled during transcription, replication and repair by anti-silencing function 1 (Asf1), a highly conserved histone chaperone. Transcription and DNA replication are also affected by histone modifications that modify nucleosome dynamics, such as H2B ubiquitylation (H2Bub). We report here that H2Bub and Asf1 cooperatively promote transcriptional silencing at yeast telomeres and mating loci. Through real time monitoring of HML (Hidden MAT Left) locus silencing, we found that transcriptional repression was slowly initiated and never fully established in mutants lacking both Asf1 and H2Bub. These findings are consistent with impaired HML silencer-binding and spreading of repressor proteins, Sir2 and Sir3. In addition, mutants lacking H2Bub and Asf1 show defects in both nucleosome assembly and higher-order heterochromatin organization at the HML locus. Our findings reveal a novel role for H2Bub and Asf1 in epigenetic silencing at mating loci. Thus, the interplay between H2Hbub and Asf1 may fine-tune nucleosome dynamics and SIR protein recruitment, and represent an ongoing requirement for proper formation and maintenance of heterochromatin.
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