ArticleTransplantation2017
The Use of an Acellular Oxygen Carrier in a Human Liver Model of Normothermic Machine Perfusion.
Article in Transplantation, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 108 citations in OpenAlex.
- Reduction in Red Blood Cell Lysis by Polymer Intervention During Rodent Liver Normothermic Machine Perfusion.Transplantation direct · 2026Article
- Comparison of Normothermic and Subnormothermic Machine Perfusion of Porcine Kidneys Using a Novel Fully Synthetic Perfusion Solution: A Proof-of-Concept Study.Journal of clinical medicine · 2026Article
- Perfluorocarbon Nanoemulsions for Simultaneous Delivery of Oxygen and Antioxidants During Machine Perfusion Supported Organ Preservation.Pharmaceutics · 2026Article
- Hemoglobin-based oxygen carriers: evolution, translational applications and clinical considerations.Frontiers in medicine · 2026Review
- Protective strategies and therapeutic interventions for ischemia-reperfusion injury: potential applications in normothermic machine perfusion.Annals of medicine and surgery (2012) · 2025Review
- Beyond Static Cold Storage: Toward the Next Generation of Tailored Organ Preservation Solutions.International journal of molecular sciences · 2025Review
- Therapeutic delivery of oxygen using artificial oxygen carriers demonstrates the possibility of treating a wide range of diseases.Journal of nanobiotechnology · 2025Review
- Organ preservation: current limitations and optimization approaches.Frontiers in medicine · 2025Review
- Acellular Perfusate is an Adequate Alternative to Packed Red Blood Cells During Normothermic Human Kidney Perfusion.Transplantation direct · 2024Article
- Oxygen Nanocarriers for Improving Cardioplegic Solution Performance: Physico-Chemical Characterization.International journal of molecular sciences · 2023Article
- Biocompatibility of the oxygen carrier polymerized human hemoglobin towards HepG2/C3A cells.Heliyon · 2023Article
- Oxygen carriers affect kidney immunogenicity duringFrontiers in transplantation · 2023Article
- Review of machine perfusion studies in vascularized composite allotransplant preservation.Frontiers in transplantation · 2023Review
- Article
- Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano-LC Coupled MALDI MS/MS.Molecules (Basel, Switzerland) · 2022Article
- Hemoglobin-based oxygen carriers: Clinical application of HBOC-201 as an alternative to red blood cells for machine perfusion in liver transplantation.Surgery in practice and science · 2022Review
- Sequential hypothermic and normothermic machine perfusion enables safe transplantation of high-risk donor livers.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Observational
- Recent Methods of Kidney Storage and Therapeutic Possibilities of Transplant Kidney.Biomedicines · 2022Review
- Article
- Optimization of Ex Vivo Machine Perfusion and Transplantation of Vascularized Composite Allografts.The Journal of surgical research · 2022Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNormothermic machine perfusion of the liver (NMP-L) is a novel technique that preserves liver grafts under near-physiological conditions while maintaining their normal metabolic activity. This process requires an adequate oxygen supply, typically delivered by packed red blood cells (RBC). We present the first experience using an acellular hemoglobin-based oxygen carrier (HBOC) Hemopure in a human model of NMP-L.
methodsFive discarded high-risk human livers were perfused with HBOC-based perfusion fluid and matched to 5 RBC-perfused livers. Perfusion parameters, oxygen extraction, metabolic activity, and histological features were compared during 6 hours of NMP-L. The cytotoxicity of Hemopure was also tested on human hepatic primary cell line cultures using an in vitro model of ischemia reperfusion injury.
resultsThe vascular flow parameters and the perfusate lactate clearance were similar in both groups. The HBOC-perfused livers extracted more oxygen than those perfused with RBCs (O2 extraction ratio 13.75 vs 9.43 % ×10 per gram of tissue, P = 0.001). In vitro exposure to Hemopure did not alter intracellular levels of reactive oxygen species, and there was no increase in apoptosis or necrosis observed in any of the tested cell lines. Histological findings were comparable between groups. There was no evidence of histological damage caused by Hemopure.
conclusionsHemopure can be used as an alternative oxygen carrier to packed red cells in NMP-L perfusion fluid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.