Evidence map›Paper›PMID 28518476›Full record

ArticleVeterinary clinical pathology2017

Adult-onset, chronic, cyclic thrombocytopenia in a Rhesus macaque (Macaca mulatta) after dengue virus vaccination and viral challenge.

Galit H Frydman, Kelly A Metcalf Pate, Robert P Marini, Armelle M de Laforcade, Irene Bosch, Vasudevan Bakthavatchalu, Sureshkumar Muthupalani, Alton G Swennes, Claire E Lyons, Ronald G Tompkins and 1 more

Open access · bronzeAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary clinical pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 1 country.

Galit H FrydmanDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Kelly A Metcalf PateDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Robert P MariniDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Armelle M de LaforcadeEmergency and Critical Care, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA.
Irene BoschInstitute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
Vasudevan BakthavatchaluDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Sureshkumar MuthupalaniDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Alton G SwennesDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Claire E LyonsDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ronald G TompkinsCenter for Surgery, Innovation & Bioengineering, Massachusetts General Hospital, Boston, MA, USA.
James G FoxDivision of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA.
Massachusetts Institute of Technology · USHarvard University · USIcahn School of Medicine at Mount Sinai · USJohns Hopkins Medicine · USJohns Hopkins University · USMassachusetts General Hospital · USTufts University · US

Funding

Biomedical Research Training for Veterinary ScientistsT32OD010978 · OD · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI FOX, JAMES G · 2012 to 2017
$2.2M
The Role of Platelet-Monocyte Interactions in SIV InfectionK01OD018244 · OD · JOHNS HOPKINS UNIVERSITY · PI PATE, KELLY ANN METCALF · 2014 to 2018
$657k
NIH HHS K01 OD018244NIH HHS T32 OD010978
6 · The paper itself

Abstract

An 8-year-old, male Rhesus macaque (Macaca mulatta), previously used for dengue virus (DENV) vaccine research with viral challenge, was presented with adult-onset, chronic, cyclic thrombocytopenia. Platelet number, morphology, and function were evaluated by automated hematology, peripheral blood smears, electron microscopy, flow cytometry, and impedance aggregometry. Bone marrow was evaluated by cytology. Both serum anti-dengue nonstructural protein 1 (NS1) antibodies and anti-platelet antibodies were detected by ELISA. Platelet characterization showed a lack of aggregation to all agonists (ADP, ASP, and collagen), increased activation with increased expression of surface marker (HLA-ABC), and an absence of surface receptor GPIX during clinical episodes of petechiae and ecchymoses, even in the presence of normal platelet counts. Bone marrow aspirates identified potential mild megakaryocytic hypoplasia. All platelet functions and morphologic attributes were within normal limits during clinically normal phases. Presence of anti-dengue NS1 serum antibodies confirmed a positive DENV titer 8 years postvaccination. Based on the history and clinical findings, a primary differential diagnosis for this chronic, cyclic platelet pathology was autoimmune platelet destruction with potential bone marrow involvement.

Indexed as

AnimalsAntibodies, ViralBlood PlateletsDengue VaccinesEnzyme-Linked Immunosorbent AssayFlow CytometryMacaca mulattaMaleMicroscopy, ElectronMonkey DiseasesPlatelet AggregationThrombocytopeniaAntibodies, ViralDengue VaccinesAutoimmuneITPnonhuman primateplatelets

Identifiers

PMID28518476
PMCPMC5977396
OpenAlexW2615175082

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.