Evidence map›Paper›PMID 28518112›Full record

ArticleJournal of visualized experiments : JoVE2017

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish.

Elaine L Welch, Celeste C Eno, Sreelaja Nair, Robin E Lindeman, Francisco Pelegri

Open access · greenAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Elaine L WelchLaboratory of Genetics, University of Wisconsin-Madison.
Celeste C EnoLaboratory of Genetics, University of Wisconsin-Madison.
Sreelaja NairDepartment of Biological Sciences, Tata Institute of Fundamental Research.
Robin E LindemanDepartment of Genetics, Cell Biology, and Development, University of Minnesota.
Francisco PelegriLaboratory of Genetics, University of Wisconsin-Madison; fjpelegri@wisc.edu.
University of Wisconsin–Madison · USTata Institute of Fundamental Research · INUniversity of Minnesota · US

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007133 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 1985 to 2023
$16.5M
Diversity Supplement: Cytokinesis and RNA segregation in zebrafish developmentR01GM065303 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PELEGRI, FRANCISCO J · 2002 to 2023
$4.7M
Cytokinesis and RNA Segregation in Zebrafish DevelopmentR56GM065303 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PELEGRI, FRANCISCO J · 2007 to 2007
$254k
Germ Plasm Aggregation and Compaction in the Early Zebrafish EmbryoF31GM108449 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI ENO, CELESTE CHLOE · 2014 to 2016
$55k
NIGMS NIH HHS F31 GM108449NIGMS NIH HHS R01 GM065303NIGMS NIH HHS R56 GM065303NIGMS NIH HHS T32 GM007133
6 · The paper itself

Abstract

Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into the developing oocyte. Because these events rely on maternal products which typically act very soon after fertilization-that preexist inside the egg, standard approaches for expression and functional reduction involving the injection of reagents into the fertilized egg are typically ineffective. Instead, such manipulations must be performed during oogenesis, prior to or during the accumulation of maternal products. This article describes in detail a protocol for the in vitro maturation of immature zebrafish oocytes and their subsequent in vitro fertilization, yielding viable embryos that survive to adulthood. This method allows the functional manipulation of maternal products during oogenesis, such as the expression of products for phenotypic rescue and tagged construct visualization, as well as the reduction of gene function through reverse-genetics agents.

Indexed as

In Vitro Oocyte Maturation TechniquesAnimalsFemaleFertilization in VitroOocytesOogenesisZebrafish

Identifiers

PMID28518112
PMCPMC5565100
OpenAlexW2607334068

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.