ArticleBlood transfusion = Trasfusione del sangue2017
Red blood cells ageing markers: a multi-parametric analysis.
Article in Blood transfusion = Trasfusione del sangue, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 72 citations in OpenAlex.
- The Proteasome Safeguards Red Blood Cell Integrity During Storage and After Transfusion.Antioxidants (Basel, Switzerland) · 2026Article
- AI-driven digital holographic microscopy for label-free quantitative cellular analysis: toward low-cost and field-deployable platforms.Biomedical optics express · 2026Review
- The loss of peroxiredoxin 2 in mice disrupts the biochemical aging phenotype in erythrocytes.iScience · 2026Article
- Cold storage surpasses the impact of biological age and donor characteristics on red blood cell morphology classified by deep machine learning.Scientific reports · 2025Article
- Initiation of Progressive Morphological Transition Towards an Echino-Stomato-Spherocytic Phenotype by Phosphatidylserine Externalization and Its Implication in Thrombosis.International journal of molecular sciences · 2025Article
- Microvesicles from stored red blood cells induce P-selectin and von Willebrand factor release from endothelial cells via a protein kinase C-dependent mechanism.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Article
- Phosphoproteomics and morphology of stored human red blood cells treated by protein tyrosine phosphatases inhibitor.Blood advances · 2024Article
- Red blood cells: a potential delivery system.Journal of nanobiotechnology · 2023Review
- In vitro-transfusional model for red-blood-cell study: the advantage of lowering hematocrit.Blood transfusion = Trasfusione del sangue · 2023Article
- Omics Technologies in Veterinary Medicine: Literature Review and Perspectives in Transfusion Medicine.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2023Review
- The Effect of the Donor's and Recipient's Sex on Red Blood Cells Evaluated Using Transfusion Simulations.Cells · 2023Article
- The time-course linkage between hemolysis, redox, and metabolic parameters during red blood cell storage with or without uric acid and ascorbic acid supplementation.Frontiers in aging · 2023Article
- Risk Assessment of Red Cell Transfusion in Congenital Heart Disease.The Thoracic and cardiovascular surgeon · 2022Article
- Interlaboratory evaluation of a digital holographic microscopy-based assay for label-free in vitro cytotoxicity testing of polymeric nanocarriers.Drug delivery and translational research · 2022Article
- Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process.Acta pharmaceutica Sinica. B · 2022Article
- Observational
- Article
- Storage-Induced Micro-Erythrocytes Can Be Quantified and Sorted by Flow Cytometry.Frontiers in physiology · 2022Article
- Deciphering the Relationship Between Free and Vesicular Hemoglobin in Stored Red Blood Cell Units.Frontiers in physiology · 2022Article
- Effect of Rho GTPase activating protein 9 combined with preoperative ratio of platelet distribution width to platelet count on prognosis of patients with serous ovarian cancer.Translational cancer research · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRed blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 °C in saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red blood cells accumulate lesions that can be reversible or irreversible upon transfusion. The aim of the present study is to follow several parameters reflecting cell metabolism, antioxidant defences, morphology and membrane dynamics during storage. MATERIALS AND
methodsFive erythrocyte concentrates were followed weekly during 71 days. Extracellular glucose and lactate concentrations, total antioxidant power, as well as reduced and oxidised intracellular glutathione levels were quantified. Microvesiculation, percentage of haemolysis and haematologic parameters were also evaluated. Finally, morphological changes and membrane fluctuations were recorded using label-free digital holographic microscopy.
resultsThe antioxidant power as well as the intracellular glutathione concentration first increased, reaching maximal values after one and two weeks, respectively. Irreversible morphological lesions appeared during week 5, where discocytes began to transform into transient echinocytes and finally spherocytes. At the same time, the microvesiculation and haemolysis started to rise exponentially. After six weeks (expiration date), intracellular glutathione was reduced by 25%, reflecting increasing oxidative stress. The membrane fluctuations showed decreased amplitudes during shape transition from discocytes to spherocytes. DISCUSSION: Various types of lesions accumulated at different chemical and cellular levels during storage, which could impact their in vivo recovery after transfusion. A marked effect was observed after four weeks of storage, which corroborates recent clinical data. The prolonged follow-up period allowed the capture of deep storage lesions. Interestingly, and as previously described, the severity of the changes differed among donors.
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