ArticleBlood transfusion = Trasfusione del sangue2017
Acetylcholinesterase provides new insights into red blood cell ageing in vivo and in vitro.
Article in Blood transfusion = Trasfusione del sangue, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Surface-Attached Model Lipid Membranes Derived from Human Red Blood Cells.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Context-tuned strategies for marker selection precision in neuronal studies.Frontiers in neuroscience · 2026Review
- The interactions of non-functionalized polystyrene nanoparticles with human albumin and erythrocyte proteins: implications and potential consequences.Scientific reports · 2025Article
- Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance.Biosensors · 2025Article
- Galangin Triggers Eryptosis and Hemolysis Through CaInternational journal of molecular sciences · 2024Article
- Non-Neuronal Acetylcholinesterase Activity Shows Limited Utility for Early Detection of Sepsis.Biomedicines · 2023Article
- Increased Enzymatic Activity of Acetylcholinesterase Indicates the Severity of the Sterile Inflammation and Predicts Patient Outcome following Traumatic Injury.Biomolecules · 2023Article
- Cholinergic System and Its Therapeutic Importance in Inflammation and Autoimmunity.Frontiers in immunology · 2021Review
- The Redox Balance and Membrane Shedding in RBC Production, Maturation, and Senescence.Frontiers in physiology · 2021Review
- Evaluation of azamethiphos and dimethoate degradation using chlorine dioxide during water treatment.Environmental science and pollution research international · 2020Article
- Acetaldehyde-induced oxidative modifications and morphological changes in isolated human erythrocytes: an in vitro study.Environmental science and pollution research international · 2020Article
- Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences.Proteomes · 2020Article
- Gallic acid and MiADMSA reversed arsenic induced oxidative/nitrosative damage in rat red blood cells.Heliyon · 2020Article
- The Relationship Between Aggregation and Deformability of Red Blood Cells in Health and Disease.Frontiers in physiology · 2020Review
- Cumulative impact of anti-sea lice treatment (azamethiphos) on health status of Rainbow trout (Oncorhynchus mykiss, Walbaum 1792) in aquaculture.Scientific reports · 2019Article
- Effects of aged stored autologous red blood cells on human plasma metabolome.Blood advances · 2019Article
- Neuropsychiatric Disorders in Farmers Associated with Organophosphorus Pesticide Exposure in a Rural Village of Northwest México.International journal of environmental research and public health · 2019Article
- Red Blood Cell Homeostasis and Altered Vesicle Formation in Patients With Paroxysmal Nocturnal Hemoglobinuria.Frontiers in physiology · 2019Article
- Altered Expression Levels of CD59, but Not CD55, on Red Blood Cells in Stored Blood.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2019Article
- Red Blood Cell Homeostasis: Mechanisms and Effects of Microvesicle Generation in Health and Disease.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDuring its 120 days sojourn in the circulation, the red blood cell (RBC) remodels its membrane. Acetylcholinesterase (AChE) is a glycosylphosphatidylinositol (GPI)-linked enzyme that may serve as a marker for membrane processes occurring this ageing-associated remodelling process. MATERIALS AND
methodsExpression and enzymatic activity of AChE were determined on RBCs of various ages, as obtained by separation based on volume and density (ageing in vivo), and on RBCs of various times of storage in blood bank conditions (ageing in vitro), as well as on RBC-derived vesicles.
resultsDuring ageing in vivo, the enzymatic activity of AChE decreases, but not the AChE protein concentration. In contrast, neither AChE activity nor concentration show a consistent, significant decrease during ageing in vitro. CD59, another GPI-linked protein that protects against complement-induced removal, also remains constant during storage. The cellular content of the integral membrane protein glycophorin A, however, decreases with storage time in the more dense RBC fractions. The latter are enriched in echinocytes and other misshapen cells during storage. DISCUSSION: Our findings suggest that, during RBC ageing, GPI-linked proteins and integral membrane proteins are differentially sorted. Also, the vesicles that are generated in vitro show a fast and extensive loss of AChE activity, but not of AChE expression. Thus, AChE characteristics may constitute sensitive biomarkers of RBC ageing in vivo, and a source of information on the structural and functional changes that GPI-linked proteins undergo during ageing in vivo and in vitro. This information may help to understand RBC homeostasis and the effects of transfusion, especially in immunologically compromised patients.
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