ArticleNature communications2017
Improving 10-deacetylbaccatin III-10-β-O-acetyltransferase catalytic fitness for Taxol production.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Engineering of 10-Deacetylbaccatin III-10-β-O-Acetyltransferase From Taxus Species for Efficient Acetylating Non-Natural Substrates Into Taxol in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei.Plant molecular biology · 2025Article
- Post-genomic illumination of paclitaxel biosynthesis.Nature plants · 2024Review
- Structure-Guided Evolution Modulate Alcohol Oxidase to Improve Ethanol Oxidation Performance.Applied biochemistry and biotechnology · 2024Article
- Research Advances in Clinical Applications, Anticancer Mechanism, Total Chemical Synthesis, Semi-Synthesis and Biosynthesis of Paclitaxel.Molecules (Basel, Switzerland) · 2023Review
- Biosynthesis and biotechnological production of the anti-obesity agent celastrol.Nature chemistry · 2023Article
- Elucidation of Geniposide and Crocin Accumulation and Their Biosysnthsis-Related Key Enzymes duringPlants (Basel, Switzerland) · 2023Article
- Targeted control of supporting pathways in paclitaxel biosynthesis with CRISPR-guided methylation.Frontiers in bioengineering and biotechnology · 2023Article
- TcJAV3-TcWRKY26 Cascade Is a Missing Link in the Jasmonate-Activated Expression of Taxol Biosynthesis GeneInternational journal of molecular sciences · 2022Article
- Impact of novel microbial secondary metabolites on the pharma industry.Applied microbiology and biotechnology · 2022Review
- Harnessing Interactional Sensory Genes for Rationally Reprogramming Chaotic Metabolism.Research (Washington, D.C.) · 2022Article
- Construction of acetyl-CoA and DBAT hybrid metabolic pathway for acetylation of 10-deacetylbaccatin III to baccatin III.Acta pharmaceutica Sinica. B · 2021Article
- Evolution-aided engineering of plant specialized metabolism.aBIOTECH · 2021Review
- Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated byMolecules (Basel, Switzerland) · 2021Review
- Microbial Cell Factory of Baccatin III Preparation inFrontiers in microbiology · 2021Article
- Recent Advances in Metabolic Engineering, Protein Engineering, and Transcriptome-Guided Insights Toward Synthetic Production of Taxol.Frontiers in bioengineering and biotechnology · 2021Review
- Evaluation of the anticancer activity of enzymatically synthesized Baccatin III: an intermediate precursor of Taxol®.3 Biotech · 2020Article
- Combinatorial mutation on theActa pharmaceutica Sinica. B · 2019Article
- Transcriptome analyses provide insights into the expression pattern and sequence similarity of several taxol biosynthesis-related genes in three Taxus species.BMC plant biology · 2019Article
- Comparative Genomic Analysis Reveals the Mechanism Driving the Diversification of Plastomic Structure in Taxaceae Species.Frontiers in genetics · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The natural concentration of the anticancer drug Taxol is about 0.02% in yew trees, whereas that of its analogue 7-β-xylosyl-10-deacetyltaxol is up to 0.5%. While this compound is not an intermediate in Taxol biosynthetic route, it can be converted into Taxol by de-glycosylation and acetylation. Here, we improve the catalytic efficiency of 10-deacetylbaccatin III-10-O-acetyltransferase (DBAT) of Taxus towards 10-deacetyltaxol, a de-glycosylated derivative of 7-β-xylosyl-10-deacetyltaxol to generate Taxol using mutagenesis. We generate a three-dimensional structure of DBAT and identify its active site using alanine scanning and design a double DBAT mutant (DBAT
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.